Loading…

The compound effect of irradiation and familial pseudohyperkalemia on potassium leak from red blood cells

Background Familial pseudohyperkalemia (FP) is a rare asymptomatic condition characterized by an increased rate of potassium leak from red blood cells (RBC) on refrigeration. Gamma irradiation compromises RBC membrane integrity and accelerates potassium leakage. Here, we compared the effect of irrad...

Full description

Saved in:
Bibliographic Details
Published in:Transfusion (Philadelphia, Pa.) Pa.), 2022-12, Vol.62 (12), p.2587-2595
Main Authors: Meli, Athinoula, Linger, Rachel, Stevens‐Hernandez, Christian J., Gyongyver, Gyorffy, Marks, Denese C., Aung, Htet Htet, Tan, Joanne C. G., Cardigan, Rebecca, Bruce, Lesley J., New, Helen V.
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Familial pseudohyperkalemia (FP) is a rare asymptomatic condition characterized by an increased rate of potassium leak from red blood cells (RBC) on refrigeration. Gamma irradiation compromises RBC membrane integrity and accelerates potassium leakage. Here, we compared the effect of irradiation, applied early or late in storage, on FP versus non‐FP RBC. Study Design Five FP and 10 non‐FP individuals from the National Institute for Health Research Cambridge BioResource, UK, and three FP and six non‐FP individuals identified by Australian Red Cross Lifeblood consented to the study. Blood was collected according to standard practice in each center, held overnight at 18–24°C, leucocyte‐depleted, and processed into red cell concentrates (RCC) in Saline Adenine Glucose Mannitol. On Day 1, RCC were split equally into six Red Cell Splits (RCS). Two RCS remained non‐irradiated, two were irradiated on Day 1 and two were irradiated on Day 14. RBCs were tested over cold storage for quality parameters. Results As expected, non‐irradiated FP RCS had significantly higher supernatant potassium levels than controls throughout 28 days of storage (p 
ISSN:0041-1132
1537-2995
DOI:10.1111/trf.17159