Eye flukes (Diplostomum spp) damage retinal tissue and may cause a regenerative response in wild threespine stickleback fish

• Published in: Experimental eye research 2022-12, Vol.225, p.109298-109298, Article 109298
• Main Authors: Frey, Ruth A., Barrett, Lindsey M., Parkin, Lauren, Blakeley, Brittany, Ålund, Murielle, Byford, Gregory, Euhus, Abigail, Tsarnas, Christine, Boughman, Janette W., Stenkamp, Deborah L.
• Format: Article
• Language: English
• Subjects: Animals; Damage; Diplostomum; Epithelium; Eye; Proliferation; Regeneration; Retina; Retina - metabolism; RPE; Smegmamorpha; Stickleback; Trematoda; Zebrafish
• ISSN: 0014-4835; 1096-0007
• DOI: 10.1016/j.exer.2022.109298

Fish rely upon vision as a dominant sensory system for foraging, predator avoidance, and mate selection. Damage to the visual system, in particular to the neural retina of the eye, has been demonstrated to result in a regenerative response in captive fish that serve as model organisms (e.g. zebrafish), and this response restores some visual function. The purpose of the present study is to determine whether damage to the visual system that occurs in wild populations of fish also results in a regenerative response, offering a potentially ecologically relevant model of retinal regeneration. Adult threespine stickleback were collected from several water bodies of Iceland, and cryosectioned eye tissues were processed for hematoxylin and eosin staining or for indirect immunofluorescence using cell-specific markers. In many of the samples, eye flukes (metacercariae of Diplostomum spp) were present, frequently between the neural retina and retinal pigmented epithelium (RPE). Damage to the retina and to the RPE was evident in eyes containing flukes, and RPE fragments were observed within fluke bodies, suggesting they had consumed this eye tissue. Expression of a cell proliferation marker was also observed in both retina and RPE, consistent with a proliferative response to the damage. Interestingly, some regions of infected retina displayed “laminar fusions,” in which neuronal cell bodies were misplaced within the major synaptic layer of the retina. These laminar fusions are also frequently found in regenerated zebrafish retina following non-parasitic (experimental) forms of retinal damage. The stickleback retina may therefore respond to fluke-mediated damage by engaging in retinal regeneration. •Eye fluke larvae infect eyes of wild threespine stickleback fish.•Eye flukes invade the subretinal space, causing retinal detachments.•Eye flukes damage retina/RPE, possibly in part by consuming them.•Infected stickleback retinas respond by local increases in cell proliferation.•Infected stickleback retinas show histological features of regenerated retinas.