Performance of the onstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, Thailand.(dengue virus )

Dengue is an arboviral disease that has a large effect on public health in subtropical and tropical countries. Rapid and accurate detection of dengue infection is necessary for diagnosis and disease management. We previously developed highly sensitive immunochromatographic devices, the TKK 1.sup.st...

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Bibliographic Details
Published in:Virology journal 2022-10, Vol.19 (1), p.1-169
Main Authors: Poltep, Kanaporn, Phadungsombat, Juthamas, Kosoltanapiwat, Nathamon, Hanboonkunupakarn, Borimas, Wiriyarat, Witthawat, Suwanpakdee, Sarin, Prompiram, Phirom, Nakayama, Emi E, Suzuki, Keita, Iwamoto, Hisahiko, Shioda, Tatsuo, Leaungwutiwong, Pornsawan
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Dengue fever
Dengue viruses
Diagnosis
Efficiency
Genotype & phenotype
Glycoproteins
Hospitals
Identification and classification
Immunoglobulin G
Immunoglobulin M
Infections
Laboratories
Patients
Proteins
Public health
Serotypes
Testing
Tropical diseases
Viral infections
Viruses
Zika virus
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Summary:Dengue is an arboviral disease that has a large effect on public health in subtropical and tropical countries. Rapid and accurate detection of dengue infection is necessary for diagnosis and disease management. We previously developed highly sensitive immunochromatographic devices, the TKK 1.sup.st and TKK 2.sup.nd kits, based on dengue virus (DENV) nonstructural protein 1 detection. However, these TKK kits were evaluated mainly using DENV type 2 clinical specimens collected in Bangladesh, and further validation using clinical specimens of other serotypes was needed. In the present study, one of the TKK kits, TKK 2.sup.nd, was evaluated using 10 DENV-1, 10 DENV-2, 4 DENV-3, 16 DENV-4, and 10 zika virus-infected clinical specimens collected in Bangkok, Thailand. The TKK 2.sup.nd kit successfully detected all four DENV serotypes in patient serum specimens and did not show any cross-reactivities against zika virus serum specimens. The IgM and/or IgG anti-DENV antibodies were detected in seven serum specimens, but did not seem to affect the results of antigen detection in the TKK 2.sup.nd kit. The results showed that the TKK 2.sup.nd kit successfully detected all four DENV serotypes in clinical specimens and confirmed the potential of the kit for dengue diagnosis in endemic countries.
ISSN:1743-422X
1743-422X
DOI:10.1186/s12985-022-01904-0