Loading…
Leptospira spp. strains associated with Bovine Genital Leptospirosis (BGL)
Bovine Genital Leptospirosis (BGL) is an important syndrome that leads to reproductive failures. The present study aimed to perform a molecular analysis of Leptospira spp. identified from genital and urine samples from in vivo naturally infected cows with poor reproductive performance. A total of 48...
Saved in:
Published in: | Microbial pathogenesis 2022-12, Vol.173, p.105841-105841, Article 105841 |
---|---|
Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c272t-2a3012ddaf6dcd698bbd509ee243da4bfab977f7cdc317f83c29906fb3e362043 |
---|---|
cites | cdi_FETCH-LOGICAL-c272t-2a3012ddaf6dcd698bbd509ee243da4bfab977f7cdc317f83c29906fb3e362043 |
container_end_page | 105841 |
container_issue | |
container_start_page | 105841 |
container_title | Microbial pathogenesis |
container_volume | 173 |
creator | Aymée, Luiza Di Azevedo, Maria Isabel Nogueira Borges, Ana Luiza dos Santos Baptista Carvalho-Costa, Filipe Anibal Lilenbaum, Walter |
description | Bovine Genital Leptospirosis (BGL) is an important syndrome that leads to reproductive failures. The present study aimed to perform a molecular analysis of Leptospira spp. identified from genital and urine samples from in vivo naturally infected cows with poor reproductive performance. A total of 48 cows destined for culling due to low reproductive efficiency were selected and submitted to sampling. Uterine fragments, cervicovaginal mucus (CVM), and urine were collected from all of the cows and processed for culturing and PCR. One isolate was recovered from the uterus of one cow. Other 25 animals were PCR-positive, totaling 26 positive cows. Of them, 18 animals were positive in lipL32-PCR to genital samples, while only seven animals were positive in urine. From those, sequencing of secY gene was performed. Of the 21 good sequences obtained, 16 were L. interrogans, two were L. noguchii, two were L. santarosai and one was L. borgpetersenii. In order to evaluate the genetic similarity of sequences found herein and other sequences from bovines worldwide, a phylogenetic analysis and haplotype networks were performed. Cows with reproductive failures had a significant association (p |
doi_str_mv | 10.1016/j.micpath.2022.105841 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2730316971</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0882401022004545</els_id><sourcerecordid>2730316971</sourcerecordid><originalsourceid>FETCH-LOGICAL-c272t-2a3012ddaf6dcd698bbd509ee243da4bfab977f7cdc317f83c29906fb3e362043</originalsourceid><addsrcrecordid>eNqFkEFLwzAUx4MoOKcfQchxHlpfkq5tTuKGTqXgRc8hTV5ZxtbWJJv47e3o8OrpweP_-_Pej5BbBikDlt9v0p0zvY7rlAPnw25eZuyMTBjIPGEcynMygbLkSQYMLslVCBsAkJmQE_JWYR-70Duvaej7lIbotWsD1SF0xumIln67uKaL7uBapCtsXdRb-od1wQU6W6yqu2ty0ehtwJvTnJLP56eP5UtSva9el49VYnjBY8K1AMat1U1ujc1lWdd2DhKRZ8LqrG50LYuiKYw1ghVNKQyXEvKmFihyDpmYktnY2_vua48hqp0LBrdb3WK3D4oXAgTLZcGG6HyMmuHO4LFRvXc77X8UA3V0pzbq5E4d3anR3cA9jBwOfxwcehWMw9agdR5NVLZz_zT8AjIyep4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2730316971</pqid></control><display><type>article</type><title>Leptospira spp. strains associated with Bovine Genital Leptospirosis (BGL)</title><source>ScienceDirect Journals</source><creator>Aymée, Luiza ; Di Azevedo, Maria Isabel Nogueira ; Borges, Ana Luiza dos Santos Baptista ; Carvalho-Costa, Filipe Anibal ; Lilenbaum, Walter</creator><creatorcontrib>Aymée, Luiza ; Di Azevedo, Maria Isabel Nogueira ; Borges, Ana Luiza dos Santos Baptista ; Carvalho-Costa, Filipe Anibal ; Lilenbaum, Walter</creatorcontrib><description>Bovine Genital Leptospirosis (BGL) is an important syndrome that leads to reproductive failures. The present study aimed to perform a molecular analysis of Leptospira spp. identified from genital and urine samples from in vivo naturally infected cows with poor reproductive performance. A total of 48 cows destined for culling due to low reproductive efficiency were selected and submitted to sampling. Uterine fragments, cervicovaginal mucus (CVM), and urine were collected from all of the cows and processed for culturing and PCR. One isolate was recovered from the uterus of one cow. Other 25 animals were PCR-positive, totaling 26 positive cows. Of them, 18 animals were positive in lipL32-PCR to genital samples, while only seven animals were positive in urine. From those, sequencing of secY gene was performed. Of the 21 good sequences obtained, 16 were L. interrogans, two were L. noguchii, two were L. santarosai and one was L. borgpetersenii. In order to evaluate the genetic similarity of sequences found herein and other sequences from bovines worldwide, a phylogenetic analysis and haplotype networks were performed. Cows with reproductive failures had a significant association (p < 0.05) with positive PCR of genital samples when compared to PCR of urine. None of the animals were positive for genital samples and urine simultaneously. A high diversity of leptospiral strains were found, even in animals of the same epidemiological region. Haplotype networks of L. interrogans showed clusters of sequences from the uterus and CVM with high similarity to other genital sequences originating from previous studies. L. borgpetersenii haplotype networks presented two major clusters with high similarity, even from worldwide sequences, while L. santarosai showed clusters with high genetic distances, even with all the sequences being from Brazil. This study reinforces the theory that BGL and renal infection are distinct diseases, as well as, genital samples are crucial for the diagnosis of cows with reproductive failures caused by leptospires. In addition, haplotype networks confirmed a high genetic similarity between sequences from the present study and Sejroe strains, reinforcing Sejroe strains as the main BGL agents.
•Subfertility is significantly associated with genital PCR positivity.•Sequencing demonstrated a high diversity of leptospiral strains, mostly Sejroe.•Genital samples are a lot more reliable than urine for diagnosing subfertility.•This study reinforces that BGL and renal infection are distinct diseases.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2022.105841</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Bovine ; Genital carriers ; Haplotype networks ; Leptospirosis ; lipL32 ; secY</subject><ispartof>Microbial pathogenesis, 2022-12, Vol.173, p.105841-105841, Article 105841</ispartof><rights>2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-2a3012ddaf6dcd698bbd509ee243da4bfab977f7cdc317f83c29906fb3e362043</citedby><cites>FETCH-LOGICAL-c272t-2a3012ddaf6dcd698bbd509ee243da4bfab977f7cdc317f83c29906fb3e362043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Aymée, Luiza</creatorcontrib><creatorcontrib>Di Azevedo, Maria Isabel Nogueira</creatorcontrib><creatorcontrib>Borges, Ana Luiza dos Santos Baptista</creatorcontrib><creatorcontrib>Carvalho-Costa, Filipe Anibal</creatorcontrib><creatorcontrib>Lilenbaum, Walter</creatorcontrib><title>Leptospira spp. strains associated with Bovine Genital Leptospirosis (BGL)</title><title>Microbial pathogenesis</title><description>Bovine Genital Leptospirosis (BGL) is an important syndrome that leads to reproductive failures. The present study aimed to perform a molecular analysis of Leptospira spp. identified from genital and urine samples from in vivo naturally infected cows with poor reproductive performance. A total of 48 cows destined for culling due to low reproductive efficiency were selected and submitted to sampling. Uterine fragments, cervicovaginal mucus (CVM), and urine were collected from all of the cows and processed for culturing and PCR. One isolate was recovered from the uterus of one cow. Other 25 animals were PCR-positive, totaling 26 positive cows. Of them, 18 animals were positive in lipL32-PCR to genital samples, while only seven animals were positive in urine. From those, sequencing of secY gene was performed. Of the 21 good sequences obtained, 16 were L. interrogans, two were L. noguchii, two were L. santarosai and one was L. borgpetersenii. In order to evaluate the genetic similarity of sequences found herein and other sequences from bovines worldwide, a phylogenetic analysis and haplotype networks were performed. Cows with reproductive failures had a significant association (p < 0.05) with positive PCR of genital samples when compared to PCR of urine. None of the animals were positive for genital samples and urine simultaneously. A high diversity of leptospiral strains were found, even in animals of the same epidemiological region. Haplotype networks of L. interrogans showed clusters of sequences from the uterus and CVM with high similarity to other genital sequences originating from previous studies. L. borgpetersenii haplotype networks presented two major clusters with high similarity, even from worldwide sequences, while L. santarosai showed clusters with high genetic distances, even with all the sequences being from Brazil. This study reinforces the theory that BGL and renal infection are distinct diseases, as well as, genital samples are crucial for the diagnosis of cows with reproductive failures caused by leptospires. In addition, haplotype networks confirmed a high genetic similarity between sequences from the present study and Sejroe strains, reinforcing Sejroe strains as the main BGL agents.
•Subfertility is significantly associated with genital PCR positivity.•Sequencing demonstrated a high diversity of leptospiral strains, mostly Sejroe.•Genital samples are a lot more reliable than urine for diagnosing subfertility.•This study reinforces that BGL and renal infection are distinct diseases.</description><subject>Bovine</subject><subject>Genital carriers</subject><subject>Haplotype networks</subject><subject>Leptospirosis</subject><subject>lipL32</subject><subject>secY</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqFkEFLwzAUx4MoOKcfQchxHlpfkq5tTuKGTqXgRc8hTV5ZxtbWJJv47e3o8OrpweP_-_Pej5BbBikDlt9v0p0zvY7rlAPnw25eZuyMTBjIPGEcynMygbLkSQYMLslVCBsAkJmQE_JWYR-70Duvaej7lIbotWsD1SF0xumIln67uKaL7uBapCtsXdRb-od1wQU6W6yqu2ty0ehtwJvTnJLP56eP5UtSva9el49VYnjBY8K1AMat1U1ujc1lWdd2DhKRZ8LqrG50LYuiKYw1ghVNKQyXEvKmFihyDpmYktnY2_vua48hqp0LBrdb3WK3D4oXAgTLZcGG6HyMmuHO4LFRvXc77X8UA3V0pzbq5E4d3anR3cA9jBwOfxwcehWMw9agdR5NVLZz_zT8AjIyep4</recordid><startdate>202212</startdate><enddate>202212</enddate><creator>Aymée, Luiza</creator><creator>Di Azevedo, Maria Isabel Nogueira</creator><creator>Borges, Ana Luiza dos Santos Baptista</creator><creator>Carvalho-Costa, Filipe Anibal</creator><creator>Lilenbaum, Walter</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202212</creationdate><title>Leptospira spp. strains associated with Bovine Genital Leptospirosis (BGL)</title><author>Aymée, Luiza ; Di Azevedo, Maria Isabel Nogueira ; Borges, Ana Luiza dos Santos Baptista ; Carvalho-Costa, Filipe Anibal ; Lilenbaum, Walter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-2a3012ddaf6dcd698bbd509ee243da4bfab977f7cdc317f83c29906fb3e362043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Bovine</topic><topic>Genital carriers</topic><topic>Haplotype networks</topic><topic>Leptospirosis</topic><topic>lipL32</topic><topic>secY</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aymée, Luiza</creatorcontrib><creatorcontrib>Di Azevedo, Maria Isabel Nogueira</creatorcontrib><creatorcontrib>Borges, Ana Luiza dos Santos Baptista</creatorcontrib><creatorcontrib>Carvalho-Costa, Filipe Anibal</creatorcontrib><creatorcontrib>Lilenbaum, Walter</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aymée, Luiza</au><au>Di Azevedo, Maria Isabel Nogueira</au><au>Borges, Ana Luiza dos Santos Baptista</au><au>Carvalho-Costa, Filipe Anibal</au><au>Lilenbaum, Walter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leptospira spp. strains associated with Bovine Genital Leptospirosis (BGL)</atitle><jtitle>Microbial pathogenesis</jtitle><date>2022-12</date><risdate>2022</risdate><volume>173</volume><spage>105841</spage><epage>105841</epage><pages>105841-105841</pages><artnum>105841</artnum><issn>0882-4010</issn><eissn>1096-1208</eissn><abstract>Bovine Genital Leptospirosis (BGL) is an important syndrome that leads to reproductive failures. The present study aimed to perform a molecular analysis of Leptospira spp. identified from genital and urine samples from in vivo naturally infected cows with poor reproductive performance. A total of 48 cows destined for culling due to low reproductive efficiency were selected and submitted to sampling. Uterine fragments, cervicovaginal mucus (CVM), and urine were collected from all of the cows and processed for culturing and PCR. One isolate was recovered from the uterus of one cow. Other 25 animals were PCR-positive, totaling 26 positive cows. Of them, 18 animals were positive in lipL32-PCR to genital samples, while only seven animals were positive in urine. From those, sequencing of secY gene was performed. Of the 21 good sequences obtained, 16 were L. interrogans, two were L. noguchii, two were L. santarosai and one was L. borgpetersenii. In order to evaluate the genetic similarity of sequences found herein and other sequences from bovines worldwide, a phylogenetic analysis and haplotype networks were performed. Cows with reproductive failures had a significant association (p < 0.05) with positive PCR of genital samples when compared to PCR of urine. None of the animals were positive for genital samples and urine simultaneously. A high diversity of leptospiral strains were found, even in animals of the same epidemiological region. Haplotype networks of L. interrogans showed clusters of sequences from the uterus and CVM with high similarity to other genital sequences originating from previous studies. L. borgpetersenii haplotype networks presented two major clusters with high similarity, even from worldwide sequences, while L. santarosai showed clusters with high genetic distances, even with all the sequences being from Brazil. This study reinforces the theory that BGL and renal infection are distinct diseases, as well as, genital samples are crucial for the diagnosis of cows with reproductive failures caused by leptospires. In addition, haplotype networks confirmed a high genetic similarity between sequences from the present study and Sejroe strains, reinforcing Sejroe strains as the main BGL agents.
•Subfertility is significantly associated with genital PCR positivity.•Sequencing demonstrated a high diversity of leptospiral strains, mostly Sejroe.•Genital samples are a lot more reliable than urine for diagnosing subfertility.•This study reinforces that BGL and renal infection are distinct diseases.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.micpath.2022.105841</doi><tpages>1</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0882-4010 |
ispartof | Microbial pathogenesis, 2022-12, Vol.173, p.105841-105841, Article 105841 |
issn | 0882-4010 1096-1208 |
language | eng |
recordid | cdi_proquest_miscellaneous_2730316971 |
source | ScienceDirect Journals |
subjects | Bovine Genital carriers Haplotype networks Leptospirosis lipL32 secY |
title | Leptospira spp. strains associated with Bovine Genital Leptospirosis (BGL) |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T16%3A48%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Leptospira%20spp.%20strains%20associated%20with%20Bovine%20Genital%20Leptospirosis%20(BGL)&rft.jtitle=Microbial%20pathogenesis&rft.au=Aym%C3%A9e,%20Luiza&rft.date=2022-12&rft.volume=173&rft.spage=105841&rft.epage=105841&rft.pages=105841-105841&rft.artnum=105841&rft.issn=0882-4010&rft.eissn=1096-1208&rft_id=info:doi/10.1016/j.micpath.2022.105841&rft_dat=%3Cproquest_cross%3E2730316971%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c272t-2a3012ddaf6dcd698bbd509ee243da4bfab977f7cdc317f83c29906fb3e362043%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2730316971&rft_id=info:pmid/&rfr_iscdi=true |