Medial orbitofrontal cortical regulation of different aspects of Pavlovian and instrumental reward seeking

• Published in: Psychopharmacology 2023-03, Vol.240 (3), p.441-459
• Main Authors: Jenni, Nicole L., Symonds, Nicola, Floresco, Stan B.
• Format: Article
• Language: English
• Subjects: Analysis; Animal cognition; Animals; Associative learning; Behavior; Biomedical and Life Sciences; Biomedicine; Brain; Conditioned reinforcement; Conditioned stimulus; Conditioning, Operant; Cues; Evaluation; Experiments; Extinction behavior; Extinction, Psychological - physiology; Feeding behavior; Food; Neurosciences; Original Investigation; Pharmacology/Toxicology; Prefrontal Cortex; Psychiatry; Psychological aspects; Psychopharmacology; Rats; Reinforcement; Reinforcement, Psychology; Reinstatement; Reward; Reward (Psychology); γ-Aminobutyric acid
• ISSN: 0033-3158; 1432-2072
• DOI: 10.1007/s00213-022-06265-8

Rationale The medial subregion of the orbitofrontal cortex (mOFC) is thought to play an important role representing the expected outcome of a given course of action, as lesioning or inactivating this cortical region results in the adoption of choice strategies based more on observable (rather than previously learned) information. Despite this, its role in mediating basic associative learning remains to be fully clarified. Objective The present series of experiments examined the role of the mOFC in (1) Pavlovian conditioned approach, (2) conditioned reinforcement, (3) extinction, and (4) cue-induced reinstatement of food-seeking behavior. Methods Separate cohorts of rats went through Pavlovian or instrumental training. Intra-mOFC infusions of either saline or GABA agonists (to temporarily inactivate neural activity) were given prior to Pavlovian approach, conditioned reinforcement, first or second day of instrumental extinction training, or cue-induced reinstatement test days. Results mOFC inactivation increased lever-CS contacts in Pavlovian conditioned approach and (2) had no effect on conditioned reinforcement. These manipulations (3) accelerated within-session instrumental extinction during the initial extinction session, but impaired subsequent extinction learning on drug-free days. (4) mOFC inactivation induced differential effects on reinstatement that depended on baseline performance. mOFC inactivation abolished reinstatement in “Reinstater” rats (who displayed robust responding under control conditions) and robustly increased reinstatement in “Non-Reinstater” rats (who showed little reinstatement under control conditions) suggesting that individual differences in reinstatement may be supported by differences in mOFC mediated representations of expected outcomes. Conclusions These findings have important implications for understanding how the mOFC uses stimulus-outcome and action-outcome expectancies to guide behavior, and how dysfunction within this region may contribute to pathological patterns of reward seeking.