Fluorotelomer alcohols are efficiently biotransformed by Cunninghamella elegans

• Published in: Environmental science and pollution research international 2023-02, Vol.30 (9), p.23613-23623
• Main Authors: Khan, Mohd Faheem, Murphy, Cormac D.
• Format: Article
• Language: English
• Subjects: Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Cunninghamella - metabolism; Cytochrome P-450 Enzyme System - metabolism; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Fluorocarbons - metabolism; Research Article; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 1614-7499; 1614-7499
• DOI: 10.1007/s11356-022-23901-0

Cunninghamella elegans is a well-studied fungus that biotransforms a range of xenobiotics owing to impressive cytochrome P450 (CYP) activity. In this paper, we report the biotransformation of 6:2 fluorotelomer alcohol (6:2 FTOH) by the fungus, yielding a range of fluorinated products that were detectable by fluorine-19 nuclear magnetic resonance spectroscopy ( 19 F NMR), gas chromatography-mass spectrometry (GC–MS) and liquid chromatography-mass spectrometry (LC–MS). Upon incubation with the pre-grown cultures, the substrate (100 mg/L) was completely consumed within 48 h, which is faster biotransformation than other fungi that have hitherto been studied. The main metabolite formed was the 5:3 fluorotelomer carboxylic acid (5:3 FTCA), which accumulated in the culture supernatant. When the cytochrome P450 inhibitor 1-aminobenzotriazole was included in the culture flasks, there was no biotransformation of 6:2 FTOH, indicating that these enzymes are key to the catalysis. Furthermore, when exogenous 5:3 FTCA was added to the fungus, the standard biotransformation of the drug flurbiprofen was inhibited, strongly suggesting that the main fluorotelomer alcohol biotransformation product inhibits CYP activity and accounts for its accumulation.