Adaptive potential of the Mediterranean mussel Mytilus galloprovincialis to short-term environmental hypoxia

• Published in: Fish & shellfish immunology 2022-12, Vol.131, p.654-661
• Main Authors: Gostyukhina, O.L., Yu, Andreyeva A., Chelebieva, E.S., Vodiasova, E.A., Lantushenko, A.O., Kladchenko, E.S.
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - metabolism; Bivalve mollusks; Ecosystem; Gills; Gills - metabolism; Hypoxia; Mytilus - genetics; Mytilus - metabolism; Oxidative Stress; Oxygen - metabolism; Reactive Oxygen Species - metabolism; Reoxygenation; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2022.10.052

Environmental hypoxia naturally occurs in coastal ecosystems and bivalve mollusks have to frequently face fluctuations of dissolved oxygen concentrations. Exposure to hypoxia is often associated with the change of the antioxidant and functional status in bivalves, and restoration of the normal oxygen supply is considered to induce oxidative stress in tissues of mollusks. The study investigates changes in the activity of two antioxidant enzymes, catalase (CAT) and superoxide dismutase (SOD), as well as the expression level of SOD and CAT genes in gills of the Mediterranean mussel, Mytilus galloprovincialis, under exposure to low dissolved oxygen concentration (2.2 mg L−1) for 24 h and 72 h, and 24 h reoxygenation period. We also evaluated the intracellular level of reactive oxygen species (ROS), mortality and changes in mitochondrial membrane potential in hemocytes following hypoxia-reoxygenation cycle. 24 h exposure to hypoxia significantly decreased activity of both enzymes, which then recovered up to control levels at the end of 72 h experimental period for SOD and after reoxygenation for CAT. Expression of antioxidant enzyme genes was up-regulated following the 72 h hypoxic exposure period and returned to the basal normoxic level after 24 h reoxygenation. Hypoxia demonstrated a time-dependent effect on the functional state of hemocytes. The 24 h exposure period did not influence aerobic respiration of hemocytes, but prolonged hypoxia (72 h) was associated with a substantial decrease in mitochondrial membrane potential of hemocytes. The intracellular ROS level and mortality of hemocytes did not change under hypoxia. Reoxygenation period was accompanied with a significant decrease of intracellular ROS level. This study indicated that hypoxia did not induce the pronounced oxidative stress in gills and the changes in the antioxidant status were reversible within 24 h of reoxygenation. Hemolymph demonstrated a stable functional state indicating the tolerance of mussels to short-time hypoxia. [Display omitted] •Exposure to 24 h hypoxia induced a decrease in the activity of SOD and СAT in gills of mussels.•24 h hypoxia did not influence the level of mitochondrial membrane potential and spontaneous ROS production by hemocytes.•72 h hypoxia was associated with an increase of expression level of СAT and SOD genes.•72 h hypoxia induced a decrease in mitochondrial membrane potential of hemocytes.•Hypoxia-reoxygenation cycle did not induce oxidative stress in gills