Study the apoptosis and necrosis inducing of fosfomycin into associated infected urothelial tissue by extended spectrum beta lactamase positive of E. coli

• Published in: Microbial pathogenesis 2022-12, Vol.173, p.105838-105838, Article 105838
• Main Authors: Alotibi, Ibrahim, Al-Sarraj, Faisal, Albiheyri, Raed, Alghamdi, Mashail A., Nass, Nada, Bouback, Thamer, Sajer, Bayan H., Al-Zahrani, Majed, Basingab, Fatemah, Alharbi, Mona
• Format: Article
• Language: English
• Subjects: Apoptosis; ESBL; Fosfomycin and Escherichia coli; Necrosis
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2022.105838

Urinary tract infection is among the greatest prevalent infections, and it is also one of the most challenging diseases to treat because there are germs that are resistant to several drugs. Antibiotics are typically provided as the treatment; however, there is a disparity in the type of antibiotic that was being prescribed, the amount of the dosage, and the length of time that patients were required to take antibiotics, which led to the creation of multidrug-resistant infections. The objective of this research is to prescribe Fosfomycin treatment for the infection brought by the Escherichia coli bacterium and to determine whether or not it is effective. Throughout the course of this research, the antimicrobial drugs fosfomycin were factored in the equation at various points. The patients who had exhibited symptoms of urinary tract infection provided their urine for the purpose of giving a sample for the studies, which were carried out on them. The results of these studies showed that there were Fosfomycin antimicrobials that were successful in disrupting the E. coli bacteria, and the least inhibitory concentration (MIC) required for the pathogen to be vulnerable was quite low. In addition, administration of fosfomycin intravenously considerably lowers both the bacterial load and the inflammatory infiltration in the kidney and bladder, which helps to preserve the structural integrity of the kidney. •Interactions of Extended-spectrum – lactamases, ESBL, AmpC and hyperproduction K1 E coli strains with the urothelial cell line (lining the bladder) and associated pathways.•Examine receptors related to the bacterial binding to urothelial cells.•Bactericidal influence of antibiotics not presently prescribed in the KSA: Fosfomycin on intracellular bacteria and bacterial strains.•Quantify plasmid by RT PCR in bacterial strains.•Examine the apoptosis, and necrosis inducing abilities of Fosfomycin antibiotics.