Significance of rhizosphere microorganisms in reclaiming water in a CELSS

• Published in: Advances in space research 1997-01, Vol.20 (10), p.1949-1958
• Main Authors: Greene, C., Bubenheim, D.L., Wignarajah, K.
• Format: Article
• Language: English
• Subjects: Biodegradation, Environmental; Conservation of Natural Resources; Culture Media - chemistry; Detergents - analysis; Detergents - metabolism; Ecological Systems, Closed; Gram-Positive Bacteria - isolation & purification; Hydroponics; Lactuca - growth & development; Lactuca - microbiology; Life Support Systems; Plant Roots - growth & development; Plant Roots - microbiology; Surface-Active Agents - analysis; Surface-Active Agents - metabolism; Water Microbiology; Water Purification - methods
• ISSN: 0273-1177; 1879-1948
• DOI: 10.1016/S0273-1177(97)00860-0

Plant-microbe interactions, such as those of the rhizosphere, may be ideally suited for recycling water in a Controlled Ecological Life Support System (CELSS). The primary contaminant of waste hygiene water will be surfactants or soaps. We identified changes in the microbial ecology in the rhizosphere of hydroponically grown lettuce during exposure to surfactant. Six week old lettuce plants were transferred into a chamber with a recirculating hydroponic system. Microbial density and population composition were determined for the nutrient solution prior to introduction of plants and then again with plants prior to surfactant addition. The surfactant Igepon was added to the recirculating nutrient solution to a final concentration of 1.0 g L −1. Bacteria density and species diversity of the solution were monitored over a 72-h period following introduction of Igepon. Nine distinct bacterial types were identified in the rhisosphere; three species accounted for 87% of the normal rhizosphere population. Microbial cell number increased in the presence of Igepon, however species diversity declined. At the point when Igepon was degraded from solution, diversity was reduced to only two species. Igepon was found to be degraded directly by only one species found in the rhizosphere. Since surfactants are degraded from the waste hygiene water within 24 h, the potential for using rhizosphere bacteria as a waste processor in a CELSS is promising.