Natural deep eutectic solvents combined with cyclodextrins: A novel strategy for chokeberry anthocyanins extraction

• Published in: Food chemistry 2023-03, Vol.405, p.134816-134816, Article 134816
• Main Authors: Jovanović, Miloš S., Krgović, Nemanja, Radan, Milica, Ćujić-Nikolić, Nada, Mudrić, Jelena, Lazarević, Zorica, Šavikin, Katarina
• Format: Article
• Language: English
• Subjects: Anthocyanins; Aronia melanocarpa; Green extraction; Hydroxypropyl-β-cyclodextrin; Molecular dynamic; NaDES
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2022.134816

•Green NaDES-UAE was used for the anthocyanins extraction from chokeberry fruits.•Choline chloride:malic acid-based NaDES was the most promising solvent.•Major NaDES-UAE influencing factors were optimized by response surface methodology.•Cyclodextrins incorporated into NaDES preserve anthocyanins from termodegradation.•The possible extraction mechanism was explored by molecular dynamics simulation. Innovative eco-friendly methods based on natural deep eutectic solvents (NaDES) coupled with ultrasound-assisted extraction were employed for chokeberry anthocyanins extractions. Nine different NaDES composed of choline chloride as a hydrogen bond acceptor and organic acids (lactic, citric, malic), sugars (glucose, fructose), polyols (glycerol, 1,2-propanediol, sorbitol), and an amide (urea) as hydrogen bond donors were screened. Malic acid-containing NaDES was selected for optimization extraction conditions (time, temperature, water in NaDES) by response surface methodology. Optimal conditions for simultaneously maximizing the anthocyanins extraction (cyanidin-3-O-glucoside, cyanidin-3-O-galactoside, cyanidin-3-O-arabinoside, total anthocyanins) were 42.7 °C, 90 min, and 40 % (w/w) water in NaDES. In the next stage of this study, the possibility to improve anthocyanins extraction at elevated temperatures by incorporating different concentrations of hydroxypropyl-β-cyclodextrin into selected NaDES was investigated. The extraction was improved at hydroxypropyl-β-cyclodextrin concentrations up to 3 % (w/w). To clarify the interaction of NaDES components and anthocyanins, a molecular dynamic simulation was conducted.