N-glycan branching enzymes involved in cancer, Alzheimer's disease and COPD and future perspectives

Over the past 3 decades, our group has been involved in studies related to the biosynthesis of N-glycan branching and related glycosyltransferases and have purified most of these Golgi-derived enzymes to homogeneity using classical purification methods and cloned the cDNA of GnT-III, IV, V, VI and F...

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Published in:Biochemical and biophysical research communications 2022-12, Vol.633, p.68-71
Main Authors: Taniguchi, Naoyuki, Ohkawa, Yuki, Maeda, Kento, Kanto, Noriko, Johnson, Emma Lee, Harada, Yoichiro
Format: Article
Language:English
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Summary:Over the past 3 decades, our group has been involved in studies related to the biosynthesis of N-glycan branching and related glycosyltransferases and have purified most of these Golgi-derived enzymes to homogeneity using classical purification methods and cloned the cDNA of GnT-III, IV, V, VI and Fut8 except GnT-IX(Vb) which was obtained by homology cloning. Based primarily on our data, we briefly summarize the significance of three major enzymes and discuss perspectives for future studies on the occasion of Ernesto's 90th birthday celebration. •GnT-III, GnT-V and Fut8 add bisecting GlcNAc, β1,6 GlcNAc branching and core fucose, respectively and those glycans are highly involved in EMT/MET in cancer, BACE1 and oxidative stress in Alzheimer's disease and Smad phosphorylation in COPD.•Glycan targeted approach such as development of inhibitors against glycosyltransferase, and use of keratan disulfide disaccharide and its analogue are promising future perspectives in the above diseases.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2022.09.027