Effects of the density of extruded pellets on starch digestion kinetics, rumen fermentation, fiber digestibility and enteric methane production in dairy cows

• Published in: Journal of animal physiology and animal nutrition 2023-07, Vol.107 (4), p.981-994
• Main Authors: Khan, Ghulam Q., Prestløkken, Egil, Lund, Peter, Hellwing, Anne L. F., Larsen, Mogens
• Format: Article
• Language: English
• Subjects: Acetic acid; Animal Feed - analysis; Animals; Cannulae; Cattle; Dairy cattle; Density; Diet - veterinary; Dietary Fiber - analysis; Digestibility; Digestion; Duodenum - metabolism; Emission analysis; Emissions; Extrusion; Female; Fermentation; Hindgut; in vivo; Intestine; Kinetics; Lactation; Latin square design; Methane; Milk - chemistry; Nutrient deficiency; Pellets; Physical properties; Rumen; Rumen - metabolism; ruminal degradation; Small intestine; Starch; Starch - metabolism
• ISSN: 0931-2439; 1439-0396
• DOI: 10.1111/jpn.13787

Dynamics of starch digestion in dairy cows fed extruded pellets differing in physical functional properties were investigated by measuring starch digestibility, post‐prandial rumen fermentation patterns, and post‐prandial duodenal starch appearance. Additionally, starch digestion effects on neutral detergent fiber (NDF) digestibility and methane (CH4) emission were studied. Pure barley was extruded to produce three treatments having pellets of either low‐density (LD), medium‐density (MD) or high‐density (HD). The experiment was conducted in a 3 × 3 Latin square design using three lactating Danish Holstein cows fitted with ruminal, duodenal and ileal cannulas. After the allocation of experimental concentrate directly into the rumen through the rumen cannula, cows were fed a basal diet low in starch. Eight samples were collected on equal time intervals (9 h) from duodenal digesta, ileal digesta and feces (grab sample) to determine digestibility. For post‐prandial rumen fermentation patterns, four sample sets of rumen dorsal, medial and ventral fluid were taken from each cow, whereas for post‐prandial duodenal starch appearance, 14 samples of duodenal chyme were obtained from each cow relative to morning feeding of experimental concentrate at 07:00 h. Ruminal, small intestinal, hindgut and total tract digestibility of starch did not differ among treatments. Similarly, NDF digestibility and CH4 emission also remained unaffected by treatments. However, compared with the LD and MD treatments, the HD treatment showed higher acetate: propionate ratio at all positions in the rumen and a higher post‐prandial duodenal starch appearance. This indicates lower ruminal starch degradation (RSD) and higher starch flow into the small intestine for HD treatment. In conclusion, the current study indicates that pellets' physical properties can manipulate RSD, where pellets with high density and fluid stability can partly shift starch digestion from the rumen to the small intestine. Indeed, further investigations are needed.