Direct quantification of cytosolic delivery of drug nanocarriers using FlAsH-EDT2

The delivery of therapeutics across the cell membrane and into the cytoplasm is a major challenge that limits the development of new therapies. This challenge is compounded by the lack of a general assay for cytosolic delivery. Here we develop this assay based on the pro-fluorophore CrAsH-EDT2, and...

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Bibliographic Details
Published in:Nanomedicine 2023-01, Vol.47, p.102626-102626, Article 102626
Main Authors: Rotem, R., Bertolini, J.A., Salvioni, L., Barbieri, L., Rizzuto, M.A., Tinelli, V., Gori, A., Adams, S., Colombo, M., Prosperi, D.
Format: Article
Language:English
Subjects:
Cell penetration
Drug Carriers - chemistry
Drug delivery
FlAsH-EDT2
Flow cytometry
HeLa Cells
Humans
Nanoparticle Drug Delivery System
Pharmaceutical Preparations
Quantification method
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Summary:The delivery of therapeutics across the cell membrane and into the cytoplasm is a major challenge that limits the development of new therapies. This challenge is compounded by the lack of a general assay for cytosolic delivery. Here we develop this assay based on the pro-fluorophore CrAsH-EDT2, and provide cytosolic penetration results for a variety of drug delivery agents (polyethyleneimine, poly-arginine, Ferritin, poly [maleic anhydride-alt-isobutene] grafted with dodecylamine, and cationic liposomes) into HeLa and T98G cells. Our results show that this method can be widely applicable to different cells and drug delivery agents, and yield statistically robust results. We later use this method to optimize and improve a model drug delivery agent's (Ferritin) cytosolic penetration. [Display omitted]
ISSN:1549-9634
1549-9642
DOI:10.1016/j.nano.2022.102626