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Direct quantification of cytosolic delivery of drug nanocarriers using FlAsH-EDT2
The delivery of therapeutics across the cell membrane and into the cytoplasm is a major challenge that limits the development of new therapies. This challenge is compounded by the lack of a general assay for cytosolic delivery. Here we develop this assay based on the pro-fluorophore CrAsH-EDT2, and...
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| Published in: | Nanomedicine 2023-01, Vol.47, p.102626-102626, Article 102626 |
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| Main Authors: | , , , , , , , , , |
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| Language: | English |
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| container_end_page | 102626 |
| container_issue | |
| container_start_page | 102626 |
| container_title | Nanomedicine |
| container_volume | 47 |
| creator | Rotem, R. Bertolini, J.A. Salvioni, L. Barbieri, L. Rizzuto, M.A. Tinelli, V. Gori, A. Adams, S. Colombo, M. Prosperi, D. |
| description | The delivery of therapeutics across the cell membrane and into the cytoplasm is a major challenge that limits the development of new therapies. This challenge is compounded by the lack of a general assay for cytosolic delivery. Here we develop this assay based on the pro-fluorophore CrAsH-EDT2, and provide cytosolic penetration results for a variety of drug delivery agents (polyethyleneimine, poly-arginine, Ferritin, poly [maleic anhydride-alt-isobutene] grafted with dodecylamine, and cationic liposomes) into HeLa and T98G cells. Our results show that this method can be widely applicable to different cells and drug delivery agents, and yield statistically robust results. We later use this method to optimize and improve a model drug delivery agent's (Ferritin) cytosolic penetration.
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| doi_str_mv | 10.1016/j.nano.2022.102626 |
| format | article |
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[Display omitted]</description><subject>Cell penetration</subject><subject>Drug Carriers - chemistry</subject><subject>Drug delivery</subject><subject>FlAsH-EDT2</subject><subject>Flow cytometry</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Nanoparticle Drug Delivery System</subject><subject>Pharmaceutical Preparations</subject><subject>Quantification method</subject><issn>1549-9634</issn><issn>1549-9642</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLAzEUhYMoVqt_wIXM0s3UvCYzA27E1gcIItR1SG8SSZlO2mRG6L8342iXru7lcO453A-hK4JnBBNxu561qvUziilNAhVUHKEzUvA6rwWnx4ed8Qk6j3GNMSsxrk_RhAlWiBJXZ-h97oKBLtv1qu2cdaA659vM2wz2nY--cZBp07gvE_aDqkP_mQ21oEJwJsSsj679zB6b-_icL-ZLeoFOrGqiufydU_TxuFg-POevb08vD_evOaTuLudVyQSAoayojFB2VRKgpcWKV5hxwgGEELWuChCgtSW1ZsxWtirqlRG8ImyKbsbcbfC73sROblwE0zSqNb6PkpYpWRSUs2SloxWCjzEYK7fBbVTYS4LlgFKu5fCTHFDKEWU6uv7N71cbow8nf-yS4W40mPTlV2IhIzjTgtE_SKX27r_8b_SphIE</recordid><startdate>202301</startdate><enddate>202301</enddate><creator>Rotem, R.</creator><creator>Bertolini, J.A.</creator><creator>Salvioni, L.</creator><creator>Barbieri, L.</creator><creator>Rizzuto, M.A.</creator><creator>Tinelli, V.</creator><creator>Gori, A.</creator><creator>Adams, S.</creator><creator>Colombo, M.</creator><creator>Prosperi, D.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202301</creationdate><title>Direct quantification of cytosolic delivery of drug nanocarriers using FlAsH-EDT2</title><author>Rotem, R. ; Bertolini, J.A. ; Salvioni, L. ; Barbieri, L. ; Rizzuto, M.A. ; Tinelli, V. ; Gori, A. ; Adams, S. ; Colombo, M. ; Prosperi, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-48736cce2358e6afb71c27f0a4803414cc6669d85c6cddf19d33f8f859be64813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Cell penetration</topic><topic>Drug Carriers - chemistry</topic><topic>Drug delivery</topic><topic>FlAsH-EDT2</topic><topic>Flow cytometry</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Nanoparticle Drug Delivery System</topic><topic>Pharmaceutical Preparations</topic><topic>Quantification method</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rotem, R.</creatorcontrib><creatorcontrib>Bertolini, J.A.</creatorcontrib><creatorcontrib>Salvioni, L.</creatorcontrib><creatorcontrib>Barbieri, L.</creatorcontrib><creatorcontrib>Rizzuto, M.A.</creatorcontrib><creatorcontrib>Tinelli, V.</creatorcontrib><creatorcontrib>Gori, A.</creatorcontrib><creatorcontrib>Adams, S.</creatorcontrib><creatorcontrib>Colombo, M.</creatorcontrib><creatorcontrib>Prosperi, D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nanomedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rotem, R.</au><au>Bertolini, J.A.</au><au>Salvioni, L.</au><au>Barbieri, L.</au><au>Rizzuto, M.A.</au><au>Tinelli, V.</au><au>Gori, A.</au><au>Adams, S.</au><au>Colombo, M.</au><au>Prosperi, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct quantification of cytosolic delivery of drug nanocarriers using FlAsH-EDT2</atitle><jtitle>Nanomedicine</jtitle><addtitle>Nanomedicine</addtitle><date>2023-01</date><risdate>2023</risdate><volume>47</volume><spage>102626</spage><epage>102626</epage><pages>102626-102626</pages><artnum>102626</artnum><issn>1549-9634</issn><eissn>1549-9642</eissn><abstract>The delivery of therapeutics across the cell membrane and into the cytoplasm is a major challenge that limits the development of new therapies. This challenge is compounded by the lack of a general assay for cytosolic delivery. Here we develop this assay based on the pro-fluorophore CrAsH-EDT2, and provide cytosolic penetration results for a variety of drug delivery agents (polyethyleneimine, poly-arginine, Ferritin, poly [maleic anhydride-alt-isobutene] grafted with dodecylamine, and cationic liposomes) into HeLa and T98G cells. Our results show that this method can be widely applicable to different cells and drug delivery agents, and yield statistically robust results. We later use this method to optimize and improve a model drug delivery agent's (Ferritin) cytosolic penetration.
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Cell penetration Drug Carriers - chemistry Drug delivery FlAsH-EDT2 Flow cytometry HeLa Cells Humans Nanoparticle Drug Delivery System Pharmaceutical Preparations Quantification method |
| title | Direct quantification of cytosolic delivery of drug nanocarriers using FlAsH-EDT2 |
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