Gene polymorphisms and serum levels of mannose‐binding lectin in Czech patients with recurrent aphthous stomatitis: A case–control study

• Published in: Journal of oral pathology & medicine 2023-01, Vol.52 (1), p.81-90
• Main Authors: Izakovicova, Pavla, Slezakova, Simona, Jiraskova Zakostelska, Zuzana, Sistkova, Jana, Mlcuchova, Natalie, Bartova, Jirina, Petanova, Jitka, Kuklinek, Pavel, Fassmann, Antonin, Dusek, Ladislav, Izakovicova Holla, Lydie, Borilova Linhartova, Petra
• Format: Article
• Language: English
• Subjects: Aphthous stomatitis; Case-Control Studies; Czech Republic; Enzyme-linked immunosorbent assay; Gene Frequency; Gene polymorphism; Genetic Predisposition to Disease; Genotype; haplogenotype; haplotype; Haplotypes; Humans; Immunological diseases; Lectins; Mannose; Mannose-Binding Lectin; MBL; polymorphism; Polymorphism, Single Nucleotide; Population studies; recurrent aphthous stomatitis; Remission; Risk factors; Serum levels; Single-nucleotide polymorphism; Stomatitis; Stomatitis, Aphthous - genetics
• ISSN: 0904-2512; 1600-0714
• DOI: 10.1111/jop.13385

Background Recurrent aphthous stomatitis is one of the most prevalent oral mucosal immunological diseases. A recent case–control study in the Egyptian population suggested that single nucleotide polymorphism Gly54Asp (rs1800450) of the mannose‐binding lectin 2 gene might affect the mannose‐binding lectin serum level and recurrent aphthous stomatitis development. The aim of this study was to determine the distribution of six functional mannose‐binding lectin 2 gene polymorphisms and analyse their role in recurrent aphthous stomatitis susceptibility in the Czech population. Methods The study included 227 subjects; 137 healthy people and 90 patients with recurrent aphthous stomatitis. Six mannose‐binding lectin 2 gene polymorphisms (rs11003125, rs7096206, rs7095891, rs5030737, rs1800450, rs1800451) were analysed by the SNaPshot assay method, mannose‐binding lectin serum levels were determined by enzyme‐linked immunosorbent assay (ELISA) method in a subgroup of subjects (N = 87). Results No significant differences in mean of mannose‐binding lectin serum levels between healthy controls and patients with recurrent aphthous stomatitis were observed (383 ng/ml ± 249 standard deviation (SD) vs. 316 ng/ml ± 177 SD in remission phase vs. 343 ng/ml ± 254 SD in active phase; p > 0.05), also the allele and genotype frequencies of the studied mannose‐binding lectin 2 polymorphisms did not differ significantly between the two groups (p > 0.05, odds ratio (OR): 0.75–1.23). Moreover, the distribution of mannose‐binding lectin 2 haplotypes and haplogenotypes was similar in the healthy subjects and patients with recurrent aphthous stomatitis (p > 0.05, OR: 0.75–1.23). Conclusions This study did not confirm the previously reported association of the mannose‐binding lectin 2 Gly54Asp gene variant and low mannose‐binding lectin serum level as the risk factors for susceptibility to recurrent aphthous stomatitis. In addition, no significant relationships between mannose‐binding lectin 2 functional haplotypes or haplogenotypes and recurrent aphthous stomatitis were observed.