Identification and multilocus genotyping of Toxoplasma gondii isolates from congenital infection in north of Iran

Congenital toxoplasmosis can cause severe consequences in the fetus, such as spontaneous abortion which is affected by parasite strain. Also, recent studies revealed the high genetic diversity of Toxoplasma gondii . This study aims to investigate the serological status of T. gondii in pregnant women...

Full description

Saved in:
Bibliographic Details
Published in:Parasitology research (1987) 2023, Vol.122 (1), p.177-184
Main Authors: Hosseini, Seyed Abdollah, Sharif, Mehdi, Sarvi, Shahabeddin, Mirzaei, Nazanin, Abediankenari, Saeid, Arefkhah, Nasir, Amouei, Afsaneh, Gholami, Sara, Anvari, Davood, Ahmadpour, Ehsan, Javidnia, Javad, Jafar-Ramaji, Tahereh, Daryani, Ahmad
Format: Article
Language:English
Subjects:
Abortion
Abortion, Spontaneous
Animals
Antibodies, Protozoan
Biomedical and Life Sciences
Biomedicine
Congenital infection
Enzyme-linked immunosorbent assay
Female
Fetuses
Formaldehyde
Genetic diversity
Genotype
Genotypes
Genotyping
Health aspects
Humans
Immunoglobulin G
Immunoglobulin M
Immunology
Iran - epidemiology
Medical Microbiology
Microbiology
Miscarriage
Paraffin
Placenta
Pregnancy
Pregnant women
Protozoa
Seroepidemiologic Studies
Serology
Toxoplasma - genetics
Toxoplasma gondii
Toxoplasmosis
Toxoplasmosis, Animal - parasitology
Toxoplasmosis, Congenital - epidemiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Congenital toxoplasmosis can cause severe consequences in the fetus, such as spontaneous abortion which is affected by parasite strain. Also, recent studies revealed the high genetic diversity of Toxoplasma gondii . This study aims to investigate the serological status of T. gondii in pregnant women, multilocus genotyping in aborted fetuses’ tissue, and archived formalin‐fixed paraffin-embedded placenta. This study was performed on 100 pregnant women with spontaneous abortion and their aborted fetuses, and 250 of the archived placentae in Iran. The blood and tissue were examined for seroprevalence and genotype determination of T. gondii using ELISA and multilocus nested-PCR–RFLP, respectively. Anti- T. gondii IgG and IgM were detected in 68 samples (68%) and 1 (1%) out of 100 serums. Toxoplasma DNA was identified in 1 (1%) aborted fetuses’ tissue and 32 (12.8%) placenta samples. Overall, ten positive DNA samples were successfully genotyped, and five genotypes were recognized (ToxoDB#1, #2, #10, #27, and #48). The obtained results indicated congenital toxoplasmosis is a severe risk in this region. As type I is highly pathogen and can lead to severe complications, the prevention of the infection should be considered in seronegative pregnant women.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-022-07714-1