Physicochemical properties and prebiotic activities of polysaccharides from Zizyphus jujube based on different extraction techniques

• Published in: International journal of biological macromolecules 2022-12, Vol.223 (Pt A), p.663-672
• Main Authors: Zou, Xiaoqin, Xiao, Jiaxi, Chi, Jianwei, Zhang, Mingwei, Zhang, Ruifen, Jia, Xuchao, Mei, Deng, Dong, Lihong, Yi, Yang, Huang, Fei
• Format: Article
• Language: English
• Subjects: Fruit - chemistry; Jujube polysaccharide extraction; Lactobacillus; Physicochemical properties; Polysaccharides - chemistry; Prebiotic activity; Water - analysis
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2022.11.057

Zizyphus jujube polysaccharide was extracted with hot water, ultrahigh pressure, deep eutectic solvent (DES) and ultrahigh pressure-assisted DES. Comparative analyses were conducted on the yield, physicochemical properties and prebiotic activity of four polysaccharides (JP-H, JP-U, JP-D and JP-UD). The yield of JP-UD (10.42 %) was 3.3 times that of JP-H (3.12 %), and its sugar content was the highest. JP-UD possessed the lowest Mw, while JP-H possessed the highest. Four JPs were acidic pyranose and mainly composed of galacturonic acid, arabinose and galactose. NMR results demonstrated that they contained not only similar glycosidic linkage but also the specific glycosidic linkage of →4)-α-D-Glcp-(l→ appeared in JP-U and JP-UD, the esterified units of GalA and CONH2 group appeared in JP-D and JP-UD, and the Terminal β-D-Galp and →4)-α-GalpA-(1→ appeared in JP-UD. JPs showed different proliferation effects on four lactobacillus strains, among which JP-UD exhibited the strongest prebiotic activity. Zizyphus jujube polysaccharides have great potential for application in the functional food and medical industry. [Display omitted] •Established a new technique—ultrahigh pressure-assisted deep eutectic solvent (DES) extraction•Four extraction techniques were compared for Zizyphus jujube polysaccharide (JP).•Higher yield, lower Mw and higher GalA content were obtained by ultrahigh pressure or DES treatment.•First reported the prebiotic activity of JPs in vitro and JP-UD was the strongest due to its lowest Mw and esterified GalA.