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The expression of Oct3/4A mRNA and not its isoforms is upregulated by the HPV16 E7 oncoprotein

Purpose Oct3/4 a transcription factor is involved in maintaining the characteristics of cancer stem cells. Oct3/4 can be expressed differentially with respect to the progression of cervical cancer (CC). In addition, Oct3/4 can give rise to three isoforms by alternative splicing of the mRNA Oct3/4A,...

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Published in:Molecular biology reports 2023-02, Vol.50 (2), p.981-991
Main Authors: Gómez-Gómez, Yazmín, Organista-Nava, Jorge, Clemente-Periván, Sayuri Itzel, Lagunas-Martínez, Alfredo, Salmerón-Bárcenas, Eric Genaro, Villanueva-Morales, Daniel, Ayala-Reyna, Dania Yahaira, del Carmen Alarcón-Romero, Luz, Ortiz-Ortiz, Julio, Jiménez-López, Marco Antonio, Bello-Rios, Ciresthel, Leyva-Vázquez, Marco Antonio, Illades‑Aguiar, Berenice
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Language:English
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Summary:Purpose Oct3/4 a transcription factor is involved in maintaining the characteristics of cancer stem cells. Oct3/4 can be expressed differentially with respect to the progression of cervical cancer (CC). In addition, Oct3/4 can give rise to three isoforms by alternative splicing of the mRNA Oct3/4A, Oct3/4B and Oct3/4B1. The aim of this study was to evaluate the mRNA expression from Oct3/4A, Oct3/4B and Oct3/4B1 in low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), CC samples, and measure the effect of the HPV16 E7 oncoprotein on the mRNA expression from Oct3/4 isoforms in the C-33A cell line. Methods The expression levels of Oct3/4A, Oct3/4B and Oct3/4B1 mRNA were analyzed by reverse transcription quantitative polymerase chain reaction (RT-qPCR) in patients with LSILs, HSILs and CC. Additionally, C-33A cells that expressed the HPV16 E7 oncoprotein were established to evaluate the effect of E7 on the expression of Oct3/4 mRNA isoforms. Results Oct3/4A ( p  = 0.02), Oct3/4B ( p  = 0. 001) and Oct3/4B1 ( p  
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-022-07988-9