Molecular mechanism of di-n-butyl phthalate promotion of bladder cancer development

To determine whether di-n-butyl phthalate (DBP) promotes the occurrence of bladder cancer (BCa) and explore the action of DBP acts on BCa cells at the cellular and molecular levels. MTT and Transwell assays were used to investigate the tumorigenic actions of DBP on BCa cells. Second-generation seque...

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Published in:Toxicology in vitro 2023-02, Vol.86, p.105508-105508, Article 105508
Main Authors: Li, En-Hui, Xu, Bai-Hui, Wei, Hai-Bin, Bai, Yu-Chen, Zhang, Qi, Yu, Wei-Wen, Xu, Zhi-Hui, Qi, Xiao-Long, Zhang, Da-Hong, Wang, Heng
Format: Article
Language:English
Subjects:
Bladder cancer
Cell proliferation
DBP
FOSB
Second-generation sequencing
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Summary:To determine whether di-n-butyl phthalate (DBP) promotes the occurrence of bladder cancer (BCa) and explore the action of DBP acts on BCa cells at the cellular and molecular levels. MTT and Transwell assays were used to investigate the tumorigenic actions of DBP on BCa cells. Second-generation sequencing was used to identify differences in gene expression before and after DBP treatment. Differential gene expression was verified by q-PCR and analyzed using bioinformatics. Cells were transfected to overexpress genes of interest and proliferation and migration were measured using MTT and Transwell assays, respectively. DBP treatment stimulated both proliferation and invasion in BCa cells. Second-generation sequencing identified differences in the expression of FOSB, JUND, ATP6V1C2, and RHOQ before and after DBP treatment. FOSB expression was confirmed by q-PCR and bioinformatic analyses. FOSB overexpression increased both proliferation and invasion in BCa cells. DBP promoted BCa tumorigenesis by inducing changes in gene expression. •This study showed that DBP promoted changes in gene expression in bladder tissue through gene regulation, upregulated the expression of FOSB and other genes, induced epithelial transformation into BCa cells, and enhanced their proliferation and migration ability, which improve our understanding of the molecular mechanisms through which DBP promotes the occurrence of BCa. These results suggest that DBP may influence the development of BCa, which improves our overall understanding of DBP toxicity and prevention awareness.
ISSN:0887-2333
1879-3177
DOI:10.1016/j.tiv.2022.105508