Quality evaluation of Syringae Folium using the five-wavelength fusion fingerprint technique combined with chemometric analysis and quantitative analysis of active constituents

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2022-12, Vol.1213, p.123520-123520, Article 123520
• Main Authors: Han, Mingshu, Li, Shuai, Li, Cun, Yang, Qian, Pang, Zhipeng, Zhai, Jianxiu, Liu, Zhihui, Li, Sikai, Han, Na, Yin, Jun
• Format: Article
• Language: English
• Subjects: Chemometric analysis; Five-wavelength fusion HPLC fingerprint; Harvesting time; Syringae Folium (SF)
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2022.123520

•The five-wavelength fusion HPLC fingerprint of Syringae Folium (SF) was first established.•The quality differences between SFs were comprehensively compared and evaluated by qualitative and quantitative analysis.•Harvesting time is the main factor affecting the quality of SF. Syringae Folium (SF) is a traditional Chinese medicine with excellent antibacterial, anti-inflammatory, and antiviral properties. It is widely distributed in northeast China and has three origins. However, the differences between the three origins have never been compared. Here, we used the five-wavelength fusion HPLC fingerprint technique combined with chemometric analysis and the comprehensive quantitative analysis of active constituents to evaluate the quality of SF from different origins, localities, and harvesting times. As a result, SF from different origins and localities showed no differences by similarity analysis, chemometric analysis, and quantitative analysis, whereas the harvesting time was found to be the key factor inducing the variation of the SF composition. In summary, the differences in origins and localities would not cause apparent disparities, while the harvest time should be considered in the SF development and application.