Stem and leaf rust–induced miRNAome in bread wheat near-isogenic lines and their comparative analysis

• Published in: Applied microbiology and biotechnology 2022-12, Vol.106 (24), p.8211-8232
• Main Authors: Nair, Minu M., Kumar, S. Hari Krishna, Jyothsna, S., Sundaram, Krishna T., Manjunatha, C., Sivasamy, M., Alagu, Manickavelu
• Format: Article
• Language: English
• Subjects: Analysis; Biomedical and Life Sciences; Biotechnology; Comparative analysis; Crop production; Cultivars; Disease; Disease resistance; Diseases and pests; Food security; Gene expression; Gene sequencing; Genes; Genetic aspects; Genomics; Growth; Health aspects; Identification and classification; Leaf rust; Leaves; Life Sciences; Methods; Microbial Genetics and Genomics; Microbiology; MicroRNA; MicroRNAs; miRNA; Properties; Proteomics; Ribonucleic acid; RNA; RNA sequencing; Stem rust; Stems; Transcriptomics; Wheat
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-022-12268-4

Wheat rusts remain a major threat to global wheat production and food security. The R -gene-mediated resistance has been employed as an efficient approach to develop rust-resistant varieties. However, evolution of new fungal races and infection strategies put forward the urgency of unravelling novel molecular players, including non-coding RNAs for plant response. This study identified microRNAs associated with Sr36 and Lr45 disease resistance genes in response to stem and leaf rust, respectively. Here, small RNA sequencing was performed on susceptible and resistant wheat near-isogenic lines inoculated with stem and leaf rust pathotypes. microRNA mining in stem rust–inoculated cultivars revealed a total of distinct 26 known and 7 novel miRNAs, and leaf rust libraries culminated with 22 known and 4 novel miRNAs. The comparative analysis between two disease sets provides a better understanding of altered miRNA profiles associated with respective R -genes and infections. Temporal differential expression pattern of miRNAs pinpoints their role during the progress of infection. Differential expression pattern of miRNAs among various treatments as well as time-course expression of miRNAs revealed stem and leaf rust–responsive miRNAs and their possible role in balancing disease resistance/susceptibility. Disclosure of guide strand, passenger strand and a variant of novel-Tae-miR02 from different subgenome origins might serve as a potential link between stem and leaf rust defence mechanisms downstream to respective R -genes. The outcome from the analysis of microRNA dynamics among two rust diseases and further characterization of identified microRNAs can contribute to significant novel insights on wheat-rust interactions and rust management. Key points • Identification and comparative analysis of stem and leaf rust–responsive miRNAs. • Chromosomal location and functional prediction of miRNAs. • Time-course expression analysis of pathogen-responsive miRNAs. Graphical abstract