Anti-inflammatory effect of green photobiomodulation in human adipose-derived mesenchymal stem cells

Photo biomodulation (PBM) as a non-invasive and safe treatment has been demonstrated the anti-inflammatory potential in a variety of cell types, including stem cells. However, further investigations using different laser parameters combined with more accurate methods such as quantitative measurement...

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Bibliographic Details
Published in:Lasers in medical science 2022-12, Vol.37 (9), p.3693-3703
Main Authors: Tamimi, Reyhaneh, Mahmoodi, Nadia Malek, Samadikhah, Hamid Reza, Tackallou, Saeed Hesami, Benisi, Soheila Zamanlui, Boroujeni, Mahdi Eskandarian
Format: Article
Language:English
Subjects:
Cell growth
Cell proliferation
Cell viability
Dentistry
Gene expression
Gene regulation
Gene sequencing
Genes
Inflammation
Irradiation
Lasers
Light therapy
Medicine
Medicine & Public Health
Mesenchymal stem cells
Monocyte chemoattractant protein 1
Optical Devices
Optics
Original Article
Parameters
Photonics
Polymerase chain reaction
Quantum Optics
Ribonucleic acid
RNA
Stem cells
Time dependence
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Summary:Photo biomodulation (PBM) as a non-invasive and safe treatment has been demonstrated the anti-inflammatory potential in a variety of cell types, including stem cells. However, further investigations using different laser parameters combined with more accurate methods such as quantitative measurement of inflammatory gene expression at the mRNA level are still necessary. The aim of this study was to evaluate the effect of 532 nm green laser on cell proliferation as well as expression of inflammatory genes in human adipose-derived mesenchymal stem cells (hADMSCs) using RNA sequencing (RNA-seq) technique and confirmatory RT-PCR. hADMSCs were cultured in DMEM low glocuse medium with 10% fetal bovine serum until the fourth passage. Cultured cells were divided in two groups: control group (no laser irradiation) and laser group, irradiated with 532 nm laser at 44 m J/cm 2 with an output power of 50 mW and a density of 6 mW/cm 2 , every other day, 7 s each time. The cell viability was assessed using MTT assay 24 h after each irradiation on days 3, 5, and 7 after cell seeding, followed by performing RNA-seq and RT-PCR. The MTT assay showed that PBM increased cell proliferation on day 5 after irradiation compared to day 3 and decreased on day 7 compared to day 5. In addition, gene expression analysis in hADMSCs using RNA-seq revealed down-regulation of inflammatory genes including CSF2, CXCL2, 3, 5, 6, 8, and CCL2, 7. These results indicate that 532 nm PBM with the parameters used in this study has a time-dependent effect on hADMSCs proliferation as well as anti-inflammatory potential. Graphical abstract
ISSN:1435-604X
0268-8921
1435-604X
DOI:10.1007/s10103-022-03654-5