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Anti-inflammatory effect of green photobiomodulation in human adipose-derived mesenchymal stem cells
Photo biomodulation (PBM) as a non-invasive and safe treatment has been demonstrated the anti-inflammatory potential in a variety of cell types, including stem cells. However, further investigations using different laser parameters combined with more accurate methods such as quantitative measurement...
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| Published in: | Lasers in medical science 2022-12, Vol.37 (9), p.3693-3703 |
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| container_end_page | 3703 |
| container_issue | 9 |
| container_start_page | 3693 |
| container_title | Lasers in medical science |
| container_volume | 37 |
| creator | Tamimi, Reyhaneh Mahmoodi, Nadia Malek Samadikhah, Hamid Reza Tackallou, Saeed Hesami Benisi, Soheila Zamanlui Boroujeni, Mahdi Eskandarian |
| description | Photo biomodulation (PBM) as a non-invasive and safe treatment has been demonstrated the anti-inflammatory potential in a variety of cell types, including stem cells. However, further investigations using different laser parameters combined with more accurate methods such as quantitative measurement of inflammatory gene expression at the mRNA level are still necessary. The aim of this study was to evaluate the effect of 532 nm green laser on cell proliferation as well as expression of inflammatory genes in human adipose-derived mesenchymal stem cells (hADMSCs) using RNA sequencing (RNA-seq) technique and confirmatory RT-PCR. hADMSCs were cultured in DMEM low glocuse medium with 10% fetal bovine serum until the fourth passage. Cultured cells were divided in two groups: control group (no laser irradiation) and laser group, irradiated with 532 nm laser at 44 m J/cm
2
with an output power of 50 mW and a density of 6 mW/cm
2
, every other day, 7 s each time. The cell viability was assessed using MTT assay 24 h after each irradiation on days 3, 5, and 7 after cell seeding, followed by performing RNA-seq and RT-PCR. The MTT assay showed that PBM increased cell proliferation on day 5 after irradiation compared to day 3 and decreased on day 7 compared to day 5. In addition, gene expression analysis in hADMSCs using RNA-seq revealed down-regulation of inflammatory genes including CSF2, CXCL2, 3, 5, 6, 8, and CCL2, 7. These results indicate that 532 nm PBM with the parameters used in this study has a time-dependent effect on hADMSCs proliferation as well as anti-inflammatory potential.
Graphical abstract |
| doi_str_mv | 10.1007/s10103-022-03654-5 |
| format | article |
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2
with an output power of 50 mW and a density of 6 mW/cm
2
, every other day, 7 s each time. The cell viability was assessed using MTT assay 24 h after each irradiation on days 3, 5, and 7 after cell seeding, followed by performing RNA-seq and RT-PCR. The MTT assay showed that PBM increased cell proliferation on day 5 after irradiation compared to day 3 and decreased on day 7 compared to day 5. In addition, gene expression analysis in hADMSCs using RNA-seq revealed down-regulation of inflammatory genes including CSF2, CXCL2, 3, 5, 6, 8, and CCL2, 7. These results indicate that 532 nm PBM with the parameters used in this study has a time-dependent effect on hADMSCs proliferation as well as anti-inflammatory potential.
Graphical abstract</description><identifier>ISSN: 1435-604X</identifier><identifier>ISSN: 0268-8921</identifier><identifier>EISSN: 1435-604X</identifier><identifier>DOI: 10.1007/s10103-022-03654-5</identifier><language>eng</language><publisher>London: Springer London</publisher><subject>Cell growth ; Cell proliferation ; Cell viability ; Dentistry ; Gene expression ; Gene regulation ; Gene sequencing ; Genes ; Inflammation ; Irradiation ; Lasers ; Light therapy ; Medicine ; Medicine & Public Health ; Mesenchymal stem cells ; Monocyte chemoattractant protein 1 ; Optical Devices ; Optics ; Original Article ; Parameters ; Photonics ; Polymerase chain reaction ; Quantum Optics ; Ribonucleic acid ; RNA ; Stem cells ; Time dependence</subject><ispartof>Lasers in medical science, 2022-12, Vol.37 (9), p.3693-3703</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-bc56359d416313938a706ce6bcd5f4d454633eded095f4317047e23b0dc9c1613</citedby><cites>FETCH-LOGICAL-c396t-bc56359d416313938a706ce6bcd5f4d454633eded095f4317047e23b0dc9c1613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Tamimi, Reyhaneh</creatorcontrib><creatorcontrib>Mahmoodi, Nadia Malek</creatorcontrib><creatorcontrib>Samadikhah, Hamid Reza</creatorcontrib><creatorcontrib>Tackallou, Saeed Hesami</creatorcontrib><creatorcontrib>Benisi, Soheila Zamanlui</creatorcontrib><creatorcontrib>Boroujeni, Mahdi Eskandarian</creatorcontrib><title>Anti-inflammatory effect of green photobiomodulation in human adipose-derived mesenchymal stem cells</title><title>Lasers in medical science</title><addtitle>Lasers Med Sci</addtitle><description>Photo biomodulation (PBM) as a non-invasive and safe treatment has been demonstrated the anti-inflammatory potential in a variety of cell types, including stem cells. However, further investigations using different laser parameters combined with more accurate methods such as quantitative measurement of inflammatory gene expression at the mRNA level are still necessary. The aim of this study was to evaluate the effect of 532 nm green laser on cell proliferation as well as expression of inflammatory genes in human adipose-derived mesenchymal stem cells (hADMSCs) using RNA sequencing (RNA-seq) technique and confirmatory RT-PCR. hADMSCs were cultured in DMEM low glocuse medium with 10% fetal bovine serum until the fourth passage. Cultured cells were divided in two groups: control group (no laser irradiation) and laser group, irradiated with 532 nm laser at 44 m J/cm
2
with an output power of 50 mW and a density of 6 mW/cm
2
, every other day, 7 s each time. The cell viability was assessed using MTT assay 24 h after each irradiation on days 3, 5, and 7 after cell seeding, followed by performing RNA-seq and RT-PCR. The MTT assay showed that PBM increased cell proliferation on day 5 after irradiation compared to day 3 and decreased on day 7 compared to day 5. In addition, gene expression analysis in hADMSCs using RNA-seq revealed down-regulation of inflammatory genes including CSF2, CXCL2, 3, 5, 6, 8, and CCL2, 7. These results indicate that 532 nm PBM with the parameters used in this study has a time-dependent effect on hADMSCs proliferation as well as anti-inflammatory potential.
Graphical abstract</description><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Cell viability</subject><subject>Dentistry</subject><subject>Gene expression</subject><subject>Gene regulation</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Inflammation</subject><subject>Irradiation</subject><subject>Lasers</subject><subject>Light therapy</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mesenchymal stem cells</subject><subject>Monocyte chemoattractant protein 1</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Original Article</subject><subject>Parameters</subject><subject>Photonics</subject><subject>Polymerase chain reaction</subject><subject>Quantum Optics</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Stem cells</subject><subject>Time dependence</subject><issn>1435-604X</issn><issn>0268-8921</issn><issn>1435-604X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kE9LxDAQxYsouK5-AU8BL16iSZOm9rgs_gPBi4K3kCbT3SxNsiatsN_erBUUD55mBn7v8eYVxTklV5SQ-jpRQgnDpCwxYaLiuDooZpSzCgvC3w5_7cfFSUobQmgtKJsVZuEHi63veuWcGkLcIeg60AMKHVpFAI-26zCE1gYXzNirwQaPrEfr0SmPlLHbkAAbiPYDDHKQwOv1zqkepQEc0tD36bQ46lSf4Ox7zovXu9uX5QN-er5_XC6esGaNGHCrK8GqxnAqGGUNu1E1ERpEq03VccMrLhgDA4Y0-Wa0JryGkrXE6EbT_M68uJx8tzG8j5AG6WzaJ1AewphkWbOaC1ELktGLP-gmjNHndJnilGZ3zjNVTpSOIaUIndxG61TcSUrkvng5FS9z8fKreFllEZtEKcN-BfHH-h_VJ-oAho0</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Tamimi, Reyhaneh</creator><creator>Mahmoodi, Nadia Malek</creator><creator>Samadikhah, Hamid Reza</creator><creator>Tackallou, Saeed Hesami</creator><creator>Benisi, Soheila Zamanlui</creator><creator>Boroujeni, Mahdi Eskandarian</creator><general>Springer 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Eskandarian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-inflammatory effect of green photobiomodulation in human adipose-derived mesenchymal stem cells</atitle><jtitle>Lasers in medical science</jtitle><stitle>Lasers Med Sci</stitle><date>2022-12-01</date><risdate>2022</risdate><volume>37</volume><issue>9</issue><spage>3693</spage><epage>3703</epage><pages>3693-3703</pages><issn>1435-604X</issn><issn>0268-8921</issn><eissn>1435-604X</eissn><abstract>Photo biomodulation (PBM) as a non-invasive and safe treatment has been demonstrated the anti-inflammatory potential in a variety of cell types, including stem cells. However, further investigations using different laser parameters combined with more accurate methods such as quantitative measurement of inflammatory gene expression at the mRNA level are still necessary. The aim of this study was to evaluate the effect of 532 nm green laser on cell proliferation as well as expression of inflammatory genes in human adipose-derived mesenchymal stem cells (hADMSCs) using RNA sequencing (RNA-seq) technique and confirmatory RT-PCR. hADMSCs were cultured in DMEM low glocuse medium with 10% fetal bovine serum until the fourth passage. Cultured cells were divided in two groups: control group (no laser irradiation) and laser group, irradiated with 532 nm laser at 44 m J/cm
2
with an output power of 50 mW and a density of 6 mW/cm
2
, every other day, 7 s each time. The cell viability was assessed using MTT assay 24 h after each irradiation on days 3, 5, and 7 after cell seeding, followed by performing RNA-seq and RT-PCR. The MTT assay showed that PBM increased cell proliferation on day 5 after irradiation compared to day 3 and decreased on day 7 compared to day 5. In addition, gene expression analysis in hADMSCs using RNA-seq revealed down-regulation of inflammatory genes including CSF2, CXCL2, 3, 5, 6, 8, and CCL2, 7. These results indicate that 532 nm PBM with the parameters used in this study has a time-dependent effect on hADMSCs proliferation as well as anti-inflammatory potential.
Graphical abstract</abstract><cop>London</cop><pub>Springer London</pub><doi>10.1007/s10103-022-03654-5</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Cell growth Cell proliferation Cell viability Dentistry Gene expression Gene regulation Gene sequencing Genes Inflammation Irradiation Lasers Light therapy Medicine Medicine & Public Health Mesenchymal stem cells Monocyte chemoattractant protein 1 Optical Devices Optics Original Article Parameters Photonics Polymerase chain reaction Quantum Optics Ribonucleic acid RNA Stem cells Time dependence |
| title | Anti-inflammatory effect of green photobiomodulation in human adipose-derived mesenchymal stem cells |
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