The addition of different oils in the diet regulates the expression of adipocytokine signaling genes in sheep longissimus dorsi muscle

• Published in: Tropical animal health and production 2022-12, Vol.54 (6), p.385-385, Article 385
• Main Authors: Souza, Paulo Henrique, Monteiro, Edwana Mara Moreira, Barbosa, Elizabeth Machado, Casseb, Alexandre Rosário, Sobrinho, Wilson Amaro Conde Moreira, Domingues, Felipe Nogueira, Faturi, Cristian, Rêgo, Aníbal Coutinho, Silva Filho, Ednaldo
• Format: Article
• Language: English
• Subjects: Adipokines - metabolism; Animal Feed - analysis; Animals; Biomedical and Life Sciences; Biosynthesis; Diet; Diet - veterinary; Fatty acids; Gene expression; Genes; Grease; Greases; Life Sciences; Male; Metabolic pathways; Muscle, Skeletal; Muscles; Palm Oil; Regular Articles; Sheep; Signaling; Soybean Oil; Soybeans; Variance analysis; Vegetable oils; Veterinary Medicine/Veterinary Science; Zoology
• ISSN: 0049-4747; 1573-7438
• DOI: 10.1007/s11250-022-03395-1

Nutrition is an important factor that regulates the expression of several genes. The aim of this work was to analyze the effects of diets containing additions of different oils on the regulation of the adipocytokine signaling gene expressions in sheep longissimus dorsi muscle. Forty males and non-neutered sheep were kept in individual cages and fed under four different treatments: control treatment (concentrate and forage) and the other three treatments containing the concentrate and forage plus 4% oil (yellow grease, soybean and palm oils). After slaughter, samples of the longissimus dorsi muscle were collected. RNA extraction followed by Real Time PCR for five adipocytokine signaling genes. ANOVA was performed followed by the Dunnett’s test (0.01). The normalized expressions of the ACLY gene were not significant between treatments to control, but for the ALDOC gene, all oil-supplemented treatments were significantly downregulated relative to the control treatment. The DUSP gene was not significantly expressed between the oil-supplemented treatments to control treatment. The ENPP1 gene was significantly upregulated with the addition of palm oil and yellow grease and the FASN gene was only significantly expressed in soybean oil-supplemented treatment to control treatment. It was concluded that the addition of different oils in the sheep diet regulated the expression of most genes for up or down, which can influence the metabolic pathways responsible for the fatty acid biosynthesis in the sheep longissimus dorsi muscle.