Purification of Lipase from Pseudomonas aeruginosa VSJK R-9 and Its Application in Combination with the Lipolytic Consortium for Bioremediation of Restaurant Wastewater

• Published in: Applied biochemistry and biotechnology 2023-03, Vol.195 (3), p.1888-1903
• Main Authors: Sutar, Vinayak P., Mali, Gajanan V., Upadhye, Vijay, Singh, Varsha K., Sinha, Rajeshwar P.
• Format: Article
• Language: English
• Subjects: Ammonium; Ammonium chloride; Ammonium sulfate; Bacteria; Biochemistry; Biodegradation; Biodegradation, Environmental; Bioremediation; Biotechnology; Chemical oxygen demand; Chemistry; Chemistry and Materials Science; Consortia; Dialysis; Edible oils; Environmental degradation; Gel electrophoresis; Humans; Lipase; Lipase - metabolism; Microorganisms; Oil pollution; Original Article; Potassium phosphate; Potassium phosphates; Pseudomonas aeruginosa; Pseudomonas aeruginosa - metabolism; Purification; Renal Dialysis; Restaurants; Sodium lauryl sulfate; Wastewater
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-022-04230-x

Microorganisms are very important in biodegradation of edible oil contaminated effluents, and they find an excellent scope in restaurant wastewater bioremediation. The edible oil in such wastewater harms the environment in a number of ways. The native bacteria in the wastewater are less effective in degrading edible oil. It leads to the formation of blockage along the sewer line. This narrows the diameter of line and forms partial to complete blockage causing overflows of wastewater exposing humans and animals to diverse pathogens. A consortium of lipolytic bacteria and lipase enzyme gives a new approach for effective and environment friendly degradation of waste oil in restaurant wastewater. In the present study, the lipase produced by Pseudomonas aeruginosa VSJK-R9 isolated from restaurant wastewater was purified by ammonium sulfate precipitation, dialysis and gel exclusion chromatography-Sephadex G-100, with 11.45-fold purification to obtain a yield of 35.08%. Its molecular mass was around 50 kDa as determined by SDS-PAGE analysis. The bioremediation of restaurant wastewater supplemented with 0.5% NH 4 Cl and 0.8% K 2 HPO 4 was studied with lipolytic consortium formed by the combination of lipolytic isolates Acinetobacter junii VSJK-R6, Pseudomonas composti  VSJK-R8 and Pseudomonas aeruginosa VSJK-R9. Further, the impact of lipase supplementation was also evaluated, and it was found that the action of consortium was boosted by lipase. The oil and chemical oxygen demand value of the restaurant wastewater was considerably decreased. These findings have shown the application of lipase for bioremediation of restaurant wastewater and its positive impact on the performance of lipolytic consortium.