Evaluation of promoter hypermethylation of tumor suppressor gene BRCA1 in epithelial ovarian cancer

Context: Epithelial ovarian cancer (EOC) is a serious gynecological issue worldwide and its late detection is the major encumbrance in treatment procedures. Hypermethylation-mediated BRCA1 gene silencing results in failure of the repair system of damaged DNA playing an important role in ovarian carc...

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Published in:Journal of cancer research and therapeutics 2022-10, Vol.18 (6), p.1578-1582
Main Authors: Das, Jhuma, Chandra, Lal, Gandhi, Gauri, Amle, Dnyanesh, Patnayak, Rachana, Khurana, Nita, Saxena, Alpana
Format: Article
Language:English
Subjects:
Care and treatment
Development and progression
Gene expression
Genetic aspects
Health aspects
Ovarian cancer
Promoters (Genetics)
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Summary:Context: Epithelial ovarian cancer (EOC) is a serious gynecological issue worldwide and its late detection is the major encumbrance in treatment procedures. Hypermethylation-mediated BRCA1 gene silencing results in failure of the repair system of damaged DNA playing an important role in ovarian carcinogenesis. BRCA1 gene hypermethylation can serve as a safe and highly specific clinical marker for EOC. Aims: The present study was conducted to evaluate the promoter hypermethylation of BRCA1 gene in EOC patients. Settings and Design: This hospital-based case-control study carried out in the tertiary care hospital in New Delhi. Subjects and Methods: Promoter hypermethylation of BRCA1 gene was examined in 30 EOC diagnosed untreated cases confirmed by histopathological examinations and compared with 30 normal healthy controls matched for age using methylation specific-polymerase chain reaction. Results: We found significantly higher BRCA1 promoter hypermethylation in the serum of EOC cases as compared to controls with P < 0.0001. BRCA1 gene methylation was found to have 70% sensitivity for the diagnosis of EOC with 100% specificity. A significant difference was observed in the range of CA125 levels, B12 and Folate levels between EOC cases and controls. Conclusions: We conclude that BRCA1 gene is significantly hypermethylated in EOC patients and thus can prove to be a noninvasive diagnostic tool. Our results provide prefatory evidence that epithelial ovarian epigenome can be influenced by dietary nutrients.
ISSN:0973-1482
1998-4138
DOI:10.4103/jcrt.JCRT_390_20