Interbacterial Chemical Communication‐Triggered Nascent Proteomics

Metabolic labeling with clickable noncanonical amino acids has enabled nascent proteome profiling, which can be performed in a cell‐type‐specific manner. However, nascent proteomics in an intercellular communication‐dependent manner remains challenging. Here we develop communication‐activated profil...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2023-01, Vol.62 (5), p.e202214010-n/a
Main Authors: Liu, Weibing, Tang, Qi, Meng, Liying, Hu, Shufan, Sun, De‐en, Li, Shan, Dai, Peng, Chen, Xing
Format: Article
Language:English
Subjects:
Amino acids
Animals
Bacteria
Bacterial Proteins - metabolism
Cell interactions
Chemical communication
Circuits
Colonization Resistance
E coli
Escherichia coli - metabolism
Indoles
Indoles - metabolism
Indoles - pharmacology
Interbacterial Communication
Labeling
Metabolic Protein Labeling
Mice
Nascent Proteomics
Proteins
Proteomes
Proteomics
Proteomics - methods
Quorum Sensing
Salmonella
Salmonella - metabolism
Tryptophan
Virulence factors
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Summary:Metabolic labeling with clickable noncanonical amino acids has enabled nascent proteome profiling, which can be performed in a cell‐type‐specific manner. However, nascent proteomics in an intercellular communication‐dependent manner remains challenging. Here we develop communication‐activated profiling of protein expression (CAPPEX), which integrates the LuxI/LuxR quorum sensing circuit with the cell‐type‐specific nascent proteomics method to enable selective click‐labeling of newly synthesized proteins in a specific bacterium upon receiving chemical signals from another reporter bacterium. CAPPEX reveals that E. coli competes with Salmonella for tryptophan as the precursor for indole, and the resulting indole suppressed the expression of virulence factors in Salmonella. This tryptophan‐indole axis confers attenuation of Salmonella invasion in host cells and living mice. The CAPPEX strategy should be widely applicable for investigating various interbacterial communication processes. The CAPPEX strategy enables communication‐triggered nascent proteomic analysis in complex bacterial populations. Applying CAPPEX, a tryptophan‐indole axis that contributes to the colonization resistance of commensal E. coli to Salmonella was discovered.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.202214010