Increased Expression of Pluripotent Factors in Human Umbilical Cord Mesenchymal Stem Cells Transfected with Three tRF Molecules

tRFs and tiRNAs are small noncoding RNA molecules that are widespread in eukaryotic and prokaryotic transcriptomes with extremely powerful functions. We screened three tRF molecules whose expression was stably elevated in reprogrammed cells by tRF and tiRNA sequencing, synthesized these three molecu...

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Bibliographic Details
Published in:Molecular biotechnology 2023-07, Vol.65 (7), p.1076-1084
Main Authors: Ruan, Guang-ping, Lin, Shu-qian, Wang, Kai, Li, Zi-an, Pan, Xing-hua
Format: Article
Language:English
Subjects:
Apoptosis
Biochemistry
Biological Techniques
Biotechnology
Cell Biology
Cell cycle
Chemical synthesis
Chemistry
Chemistry and Materials Science
Citation indexes
Gene expression
Human Genetics
Laboratories
Mesenchymal stem cells
Oct-4 protein
Original Paper
Pluripotency
Protein Science
Proteins
Stem cells
Telomeres
Transcriptomes
Transfection
Umbilical cord
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Summary:tRFs and tiRNAs are small noncoding RNA molecules that are widespread in eukaryotic and prokaryotic transcriptomes with extremely powerful functions. We screened three tRF molecules whose expression was stably elevated in reprogrammed cells by tRF and tiRNA sequencing, synthesized these three molecules and transfected them into human umbilical cord mesenchymal stem cells. We detected the pluripotent factor OCT4 by Western Blot (WB) after transfection. The gene and protein expression of the pluripotent genes OCT4 and NANOG increased significantly, and telomere (TEL) expression increased significantly. Cell activity was increased, apoptosis was decreased, and the cell cycle had also changed to some extent. These results showed that the three tRF molecules, tRF-16-K87965D (sequence: CCCGGGTTTCGGCACC), tRF-17-K879652 (sequence: CCCGGGTTTCGGCACCA), and tRF-22-WD8YQ84V2 (sequence: TCGACTCCTGGCTGGCTCGCCA), can promote cell rejuvenation and increase pluripotency.
ISSN:1073-6085
1559-0305
DOI:10.1007/s12033-022-00596-9