Evaluation of the pathogenesis of non-typical strain with α-hemolysin, Vibrio parahaemolyticus 353, isolated from Chinese seafood through comparative genome and transcriptome analysis

• Published in: Marine pollution bulletin 2023-01, Vol.186, p.114276-114276, Article 114276
• Main Authors: Zha, Fei, Pang, Rui, Huang, Shixuan, Zhang, Jumei, Wang, Juan, Chen, Moutong, Xue, Liang, Ye, Qinghua, Wu, Shi, Yang, Meiyan, Gu, Qihui, Ding, Yu, Zhang, Hao, Wu, Qingping
• Format: Article
• Language: English
• Subjects: Aquatic food; Caco-2 Cells; Comparative genome; Gene Expression Profiling; Genome-sequencing; Hemolysin Proteins - genetics; Humans; Seafood - analysis; Transcriptome; Vibrio Infections; Vibrio parahaemolyticus; Virulence factors; Virulence Factors - genetics
• ISSN: 0025-326X; 1879-3363
• DOI: 10.1016/j.marpolbul.2022.114276

Vibrio parahaemolyticus outbreaks frequently occur, causing gastrointestinal sickness owing to the consumption of aquatic foods by various virulence factors; however, the mechanism of pathogenesis is still unknown. In this study, a non-typical strain of V. parahaemolyticus, named VP353, was isolated from shrimp in China. Its comparative genome and transcriptome after infection with Caco-2 cells were examined to illustrate the mechanisms of its pathogenesis. VP353 was a tdh-trh- strain but uncommonly manifested robust cytotoxicity towards Caco-2 cells. Compared with the standard strain RIMD2210633, VP353 harbored alpha-hemolysins (hlyA, hlyB, hlyC, and hlyD) was first reported in V. parahaemolyticus and showed high diversity in the T3SS2 gene cluster. Moreover, the expression of flagella, T2SS, quorum sensing-related genes, hlyA, hlyC were up-regulated, and hlyB, hlyD were down-regulated. In summary, our results demonstrate that some novel virulence factors contribute to the pathogenesis of V. parahaemolyticus infection. •Vibrio parahaemolyticus is considered a widely spread foodborne pathogen in seafood.•V. parahaemolyticus 353 was tdh/trh negative strain and show high cytotoxicity, enterotoxicity, and high diversity in T3SS2 gene cluster.•This is the first report that V. parahaemolyticus 353 harbors hlyA, hlyB, hlyC, and hlyD.•The expression of flagella, type IV pilus, EPS T2SS, quorum sensing related genes were overexpressed after infection towards Caco-2 cells.