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Development of an Endoplasmic Reticulum-targeting Fluorescent Probe for the Imaging of Superoxide Anion in Living Cells
Superoxide anion (O 2 •− ) is an important reactive oxygen species (ROS), and plays critical roles in biological systems. ER stress has close relation with many metabolic diseases, and could lead to the abnormal production of ROS including O 2 •− . Herein, we present an ER-targeting probe ( ER-Tf )...
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| Published in: | Journal of fluorescence 2023-03, Vol.33 (2), p.509-515 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c419t-70f653d73919436ab099644810b9c262e6cd2e05a85840c86dd19afa15e299bd3 |
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| cites | cdi_FETCH-LOGICAL-c419t-70f653d73919436ab099644810b9c262e6cd2e05a85840c86dd19afa15e299bd3 |
| container_end_page | 515 |
| container_issue | 2 |
| container_start_page | 509 |
| container_title | Journal of fluorescence |
| container_volume | 33 |
| creator | Wei, Hua Wang, Yan Chen, Qingxian Sun, Yaru Yue, Tao Dong, Baoli |
| description | Superoxide anion (O
2
•−
) is an important reactive oxygen species (ROS), and plays critical roles in biological systems. ER stress has close relation with many metabolic diseases, and could lead to the abnormal production of ROS including O
2
•−
. Herein, we present an ER-targeting probe (
ER-Tf
) for the detection of O
2
•−
in living cells. The probe
ER-Tf
used triflate as the response site for O
2
•−
, and employed p-methylbenzenesulfonamide as ER-targeting moiety. In response to O
2
•−
, the triflate of the probe
ER-Tf
converted to hydroxyl group, providing strong blue emission under the excitation of ultraviolet light. The probe
ER-Tf
exhibited high sensitivity and selectivity to O
2
•−
. Bioimaging experiments showed that the probe
ER-Tf
can be applied to detect O
2
•−
at ER, and also demonstrated that rotenone could increase the generation of O
2
•−
in living cells, while the O
2
•−
level at ER showed no remarkable change during ferroptosis. |
| doi_str_mv | 10.1007/s10895-022-03079-x |
| format | article |
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2
•−
) is an important reactive oxygen species (ROS), and plays critical roles in biological systems. ER stress has close relation with many metabolic diseases, and could lead to the abnormal production of ROS including O
2
•−
. Herein, we present an ER-targeting probe (
ER-Tf
) for the detection of O
2
•−
in living cells. The probe
ER-Tf
used triflate as the response site for O
2
•−
, and employed p-methylbenzenesulfonamide as ER-targeting moiety. In response to O
2
•−
, the triflate of the probe
ER-Tf
converted to hydroxyl group, providing strong blue emission under the excitation of ultraviolet light. The probe
ER-Tf
exhibited high sensitivity and selectivity to O
2
•−
. Bioimaging experiments showed that the probe
ER-Tf
can be applied to detect O
2
•−
at ER, and also demonstrated that rotenone could increase the generation of O
2
•−
in living cells, while the O
2
•−
level at ER showed no remarkable change during ferroptosis.</description><identifier>ISSN: 1053-0509</identifier><identifier>EISSN: 1573-4994</identifier><identifier>DOI: 10.1007/s10895-022-03079-x</identifier><identifier>PMID: 36449229</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Analytical Chemistry ; Anions ; Biochemistry ; Biological and Medical Physics ; Biomedical and Life Sciences ; Biomedicine ; Biophysics ; Biotechnology ; Cells (biology) ; Endoplasmic reticulum ; Fluorescent indicators ; Hydroxyl groups ; Medical imaging ; Metabolic disorders ; Selectivity ; Ultraviolet radiation</subject><ispartof>Journal of fluorescence, 2023-03, Vol.33 (2), p.509-515</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-70f653d73919436ab099644810b9c262e6cd2e05a85840c86dd19afa15e299bd3</citedby><cites>FETCH-LOGICAL-c419t-70f653d73919436ab099644810b9c262e6cd2e05a85840c86dd19afa15e299bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36449229$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wei, Hua</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Chen, Qingxian</creatorcontrib><creatorcontrib>Sun, Yaru</creatorcontrib><creatorcontrib>Yue, Tao</creatorcontrib><creatorcontrib>Dong, Baoli</creatorcontrib><title>Development of an Endoplasmic Reticulum-targeting Fluorescent Probe for the Imaging of Superoxide Anion in Living Cells</title><title>Journal of fluorescence</title><addtitle>J Fluoresc</addtitle><addtitle>J Fluoresc</addtitle><description>Superoxide anion (O
2
•−
) is an important reactive oxygen species (ROS), and plays critical roles in biological systems. ER stress has close relation with many metabolic diseases, and could lead to the abnormal production of ROS including O
2
•−
. Herein, we present an ER-targeting probe (
ER-Tf
) for the detection of O
2
•−
in living cells. The probe
ER-Tf
used triflate as the response site for O
2
•−
, and employed p-methylbenzenesulfonamide as ER-targeting moiety. In response to O
2
•−
, the triflate of the probe
ER-Tf
converted to hydroxyl group, providing strong blue emission under the excitation of ultraviolet light. The probe
ER-Tf
exhibited high sensitivity and selectivity to O
2
•−
. Bioimaging experiments showed that the probe
ER-Tf
can be applied to detect O
2
•−
at ER, and also demonstrated that rotenone could increase the generation of O
2
•−
in living cells, while the O
2
•−
level at ER showed no remarkable change during ferroptosis.</description><subject>Analytical Chemistry</subject><subject>Anions</subject><subject>Biochemistry</subject><subject>Biological and Medical Physics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biophysics</subject><subject>Biotechnology</subject><subject>Cells (biology)</subject><subject>Endoplasmic reticulum</subject><subject>Fluorescent indicators</subject><subject>Hydroxyl groups</subject><subject>Medical imaging</subject><subject>Metabolic disorders</subject><subject>Selectivity</subject><subject>Ultraviolet radiation</subject><issn>1053-0509</issn><issn>1573-4994</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kUmP1DAQhS0EYhb4AxyQJS5cDOUtiY-jnoWRWgKxnC0nqTQZOXawk6H597jpASQOnGyrvvfqyY-QFxzecID6bebQGM1ACAYSasP2j8gp17Vkyhj1uNxBSwYazAk5y_kOAEyjmqfkRFZKGSHMKfl-iffo4zxhWGgcqAv0KvRx9i5PY0c_4jJ2q18ntri0K4-wo9d-jQlzd1B8SLFFOsREl69Ibye3OxDF59M6Y4r7sUd6EcYY6Bjodrw_TDfofX5GngzOZ3z-cJ6TL9dXnzfv2Pb9ze3mYss6xc3CahgqLftaGm6UrFwLxpTsDYfWdKISWHW9QNCu0Y2Crqn6nhs3OK5RGNP28py8PvrOKX5bMS92Gkt0713AuGYraiV1-SatCvrqH_QurimUdIWqTd1IzptCiSPVpZhzwsHOaZxc-mE52EMt9liLLbXYX7XYfRG9fLBe2wn7P5LfPRRAHoFcRmGH6e_u_9j-BPwsmK0</recordid><startdate>20230301</startdate><enddate>20230301</enddate><creator>Wei, Hua</creator><creator>Wang, Yan</creator><creator>Chen, Qingxian</creator><creator>Sun, Yaru</creator><creator>Yue, Tao</creator><creator>Dong, Baoli</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20230301</creationdate><title>Development of an Endoplasmic Reticulum-targeting Fluorescent Probe for the Imaging of Superoxide Anion in Living Cells</title><author>Wei, Hua ; Wang, Yan ; Chen, Qingxian ; Sun, Yaru ; Yue, Tao ; Dong, Baoli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-70f653d73919436ab099644810b9c262e6cd2e05a85840c86dd19afa15e299bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Analytical Chemistry</topic><topic>Anions</topic><topic>Biochemistry</topic><topic>Biological and Medical Physics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biophysics</topic><topic>Biotechnology</topic><topic>Cells (biology)</topic><topic>Endoplasmic reticulum</topic><topic>Fluorescent indicators</topic><topic>Hydroxyl groups</topic><topic>Medical imaging</topic><topic>Metabolic disorders</topic><topic>Selectivity</topic><topic>Ultraviolet radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, Hua</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Chen, Qingxian</creatorcontrib><creatorcontrib>Sun, Yaru</creatorcontrib><creatorcontrib>Yue, Tao</creatorcontrib><creatorcontrib>Dong, Baoli</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of fluorescence</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, Hua</au><au>Wang, Yan</au><au>Chen, Qingxian</au><au>Sun, Yaru</au><au>Yue, Tao</au><au>Dong, Baoli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of an Endoplasmic Reticulum-targeting Fluorescent Probe for the Imaging of Superoxide Anion in Living Cells</atitle><jtitle>Journal of fluorescence</jtitle><stitle>J Fluoresc</stitle><addtitle>J Fluoresc</addtitle><date>2023-03-01</date><risdate>2023</risdate><volume>33</volume><issue>2</issue><spage>509</spage><epage>515</epage><pages>509-515</pages><issn>1053-0509</issn><eissn>1573-4994</eissn><abstract>Superoxide anion (O
2
•−
) is an important reactive oxygen species (ROS), and plays critical roles in biological systems. ER stress has close relation with many metabolic diseases, and could lead to the abnormal production of ROS including O
2
•−
. Herein, we present an ER-targeting probe (
ER-Tf
) for the detection of O
2
•−
in living cells. The probe
ER-Tf
used triflate as the response site for O
2
•−
, and employed p-methylbenzenesulfonamide as ER-targeting moiety. In response to O
2
•−
, the triflate of the probe
ER-Tf
converted to hydroxyl group, providing strong blue emission under the excitation of ultraviolet light. The probe
ER-Tf
exhibited high sensitivity and selectivity to O
2
•−
. Bioimaging experiments showed that the probe
ER-Tf
can be applied to detect O
2
•−
at ER, and also demonstrated that rotenone could increase the generation of O
2
•−
in living cells, while the O
2
•−
level at ER showed no remarkable change during ferroptosis.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>36449229</pmid><doi>10.1007/s10895-022-03079-x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of fluorescence, 2023-03, Vol.33 (2), p.509-515 |
| issn | 1053-0509 1573-4994 |
| language | eng |
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| subjects | Analytical Chemistry Anions Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biomedicine Biophysics Biotechnology Cells (biology) Endoplasmic reticulum Fluorescent indicators Hydroxyl groups Medical imaging Metabolic disorders Selectivity Ultraviolet radiation |
| title | Development of an Endoplasmic Reticulum-targeting Fluorescent Probe for the Imaging of Superoxide Anion in Living Cells |
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