A novel method for identifying Chlamydomonas reinhardtii (Chlorophyta) and closely related species from nature

Here, we introduce a new method for efficiently sampling Chlamydomonas reinhardtii and closely related species using a colony PCR‐based screen with novel primer sets designed to specifically detect these important model microalgae. To demonstrate the utility of our new method, we collected 130 soil...

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Bibliographic Details
Published in:Journal of phycology 2023-02, Vol.59 (1), p.281-288
Main Authors: Ford, Scott A., Craig, Rory J., Ness, Rob W.
Format: Article
Language:English
Subjects:
Algae
Base Sequence
Chlamydomonas reinhardtii
Chlamydomonas reinhardtii - genetics
Colonies
Haplotypes
Identification
Introduced species
isolation techniques
Methods
novel field isolates
Nucleotide sequence
Ontario
Phylogenetics
Phylogeny
Phytoplankton
Polymerase Chain Reaction
rbcL
rRNA 18S
soil sampling
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Summary:Here, we introduce a new method for efficiently sampling Chlamydomonas reinhardtii and closely related species using a colony PCR‐based screen with novel primer sets designed to specifically detect these important model microalgae. To demonstrate the utility of our new method, we collected 130 soil samples from a wide range of habitats in Ontario, Canada and identified 33 candidate algae, which were barcoded by sequencing a region of the rbcL plastid gene. For select isolates, 18S rRNA gene and YPT4 nuclear markers were also sequenced. Based on phylogenetic and haplotype network analyses of these three loci, seven novel isolates were identified as C. reinhardtii, and one additional isolate appeared to be more closely related to C. reinhardtii than any other known species. All seven new C. reinhardtii strains were interfertile with previously collected C. reinhardtii field isolates, validating the effectiveness of our molecular screen.
ISSN:0022-3646
1529-8817
DOI:10.1111/jpy.13306