Indigenous cyanobacteria enhances remediation of arsenic-contaminated soils by regulating physicochemical properties, microbial community structure and function in soil microenvironment

• Published in: The Science of the total environment 2023-02, Vol.860, p.160543-160543, Article 160543
• Main Authors: Mao, Qing, Xie, Zuoming, Pei, Fuwen, Irshad, Sana, Issaka, Sakinatu, Randrianarison, Gilbert
• Format: Article
• Language: English
• Subjects: Arsenic; Arsenic - analysis; As metabolism genes; Biodegradation, Environmental; Carbon; Chlorophyll A - analysis; Cyanobacteria - metabolism; Cyanobacterial crust; Microbial diversity; Microbiota; Mining soil; Silicon Dioxide; Soil - chemistry; Soil Microbiology; Soil Pollutants - analysis
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2022.160543

Biocrust was widely used for the immobilization and removal of arsenic (As) in drainage systems of rice fields and mining areas. In this study, the role of an indigenous cyanobacteria (Leptolyngbya sp. XZMQ) was explored in the bioremediation of As-contaminated farmland and tailing soil. After 80 d of inoculation with cyanobacteria, total As (As(T)) accumulated in the cyanobacterial crust of farmland and tailing soil was 279.89 mg kg−1 and 269.57 mg kg−1, respectively, and non-EDTA exchangeable fraction was the major fraction of it. The As(T) in farmland and tailing soil of micro-environment decreased by 10.76% and 12.73%, respectively. Meanwhile, the available As (As(a)) decreased by 21.25% and 27.65%, respectively. The XRD results showed that hematite and SiO2 existed in cyanobacterial crust of farmland and tailing soil. FTIR spectra indicated that the adsorption of As in cyanobacterial crust was mediated by OH and CO. After inoculation of Leptolyngbya sp. XZMQ, in subcrust soil, As biotransformation gene aioA was the most abundant, followed by arsM. The dominant phyla of soil biota were Proteobacteria, Cyanobacteria, Actinobacteria, and Bacteroiota, which could play critical roles in shaping aioA and arsM harboring microbe communities in soil. Redundancy analysis (RDA) showed that soil organic carbon (OC), pH, and chlorophyll a (Chl a) were the most important environmental factors in altering soil bacterial communities. Correlation analysis showed the Leptolyngbya had a positive correlation with Chl a, effective nitrogen (N(a)), electrical conductivity (EC), OC, pH in the soil, respectively, while it had a significant negative correlation with As(a), As(III) and As(T). These results emphasized on the significance of cyanobacteria in the behavior of As in mine soils and offered a promising strategy for bioremediation of As-contaminated soil in the mining area. [Display omitted] •Cyanobacterial crust reduced the bioavailability of As in micro-scale soil layer.•Cyanobacteria alter biotic and abiotic synergism to immobilize As.•Non-EDTA-exchangeable As was immobilized on cyanobacterial crust.•High abundance of aioA and arsM in mining soil with cyanobacteria inoculation•Cyanobacteria inoculation improved the fertility of mining soil.