A peptide derived from the N-terminal of NS2A for the preparation of ZIKV NS2A recognition polyclonal antibody

Zika virus non-structural protein NS2A participates in viral replication, organization, and budding, as well as escaping host immunity. NS2A also involved in the induction of microcephaly by ZIKV. However, the above studies were mainly performed through NS2A with a tag due to the lack of available a...

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Bibliographic Details
Published in:Journal of immunological methods 2023-01, Vol.512, p.113396-113396, Article 113396
Main Authors: Yu, Yufeng, Chen, Yongkang, Wang, Jian, Fan, Xiuling, He, Zhenrui, Qiao, Shaojun, Hou, Shishi, Zou, Peng
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - metabolism
Antibody
NS2A
Peptide
Peptides - metabolism
Rabbits
Viral Nonstructural Proteins - metabolism
Virus Replication
Zika Virus
Zika Virus Infection - diagnosis
ZIKV
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Summary:Zika virus non-structural protein NS2A participates in viral replication, organization, and budding, as well as escaping host immunity. NS2A also involved in the induction of microcephaly by ZIKV. However, the above studies were mainly performed through NS2A with a tag due to the lack of available antibodies against NS2A. ZIKV NS2A is a multiplex transmembrane protein, which leads to difficulties in the preparation of its recognition antibodies, thus seriously affecting the study of ZIKV NS2A. In this study, we found that a peptide (GSTDHMDHFSLGVLC) derived from the N-terminal of ZIKV NS2A coupled to KLH induced antibodies recognizing ZIKV NS2A in rabbits. The purified polyclonal antibody recognized ZIKV NS2A in ZIKV-infected cells with high efficiency and specificity, as detected by western blot and immunofluorescence assay. Our study has important implications for the preparation of ZIKV NS2A antibodies and the in-depth study of ZIKV NS2A.
ISSN:0022-1759
1872-7905
DOI:10.1016/j.jim.2022.113396