An efficient and sustainable furfurylamine production from biomass-derived furfural by a robust mutant ω-transaminase biocatalyst

[Display omitted] •Two amino acid residues of Aspergillus terreus ω-transaminase was mutated.•The transamination activity and thermostability of TMEF were significantly improved.•Furfural (500 mM) was transformed to furfurylamine (92% yield) with TMEF after 12 h.•101.3 mM of biomass-derived furfural...

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Bibliographic Details
Published in:Bioresource technology 2023-02, Vol.369, p.128425-128425, Article 128425
Main Authors: Di, Junhua, Li, Qing, Ma, Cuiluan, He, Yu-Cai
Format: Article
Language:English
Subjects:
Biomass
Catalysis
Deep eutectic solvent
Furaldehyde - chemistry
Furans
Furfural
Furfurylamine
Lignocellulose
Transaminases - genetics
Xylose - metabolism
ω-Transaminase
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Summary:[Display omitted] •Two amino acid residues of Aspergillus terreus ω-transaminase was mutated.•The transamination activity and thermostability of TMEF were significantly improved.•Furfural (500 mM) was transformed to furfurylamine (92% yield) with TMEF after 12 h.•101.3 mM of biomass-derived furfural were fully converted to furfurylamine in 5 h.•An efficient chemoenzymatic catalysis of biomass to valuable furans was developed. Furfurylamine is a key furan-based compound for manufacturing perfumes, fibers, additives, medicines and agrochemicals. It can be obtained by amination of furfural by ω-transaminase (AtAT) from Aspergillus terreus. In this work, site-directed mutant of amino acid residues [Threonine (T) at AT130 was mutated to Methionine (M) and Glutamic acid (E) at AT133 was mutated to Phenylalanine (F)] was used to change in the flexible region of AtAT. The transamination activity and thermostability were significantly improved. In ChCl:MA (30 wt%), furfural (500 mM) was efficiently transformed into furfurylamine (92% yield) with TMEF after 12 h. 101.3 mM of biomass-derived furfural and 129.7 mM of D-xylose-derived furfural were wholly converted into furfurylamine within 5 h, achieving the productivity of 0.465 g furfurylamine/(g xylan in corncob) and 0.302 g furfurylamine/(g D-xylose). This established chemoenzymatic conversion strategy by bridging chemocatalysis and biocatalysis could be utilized in the valorisation of renewable biomass to valuable furans.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2022.128425