Daylight ultraviolet B radiation ruptured the cell membrane, promoted nucleotide metabolism and inhibited energy metabolism in the plasma of Pacific oyster

• Published in: The Science of the total environment 2023-03, Vol.862, p.160729-160729, Article 160729
• Main Authors: Song, Hong-Ce, Xie, Chao-Yi, Kong, Qing, Wei, Lei, Wang, Xiao-Tong
• Format: Article
• Language: English
• Subjects: Animals; Cell Membrane - metabolism; Cell Membrane - radiation effects; Crassostrea - physiology; Crassostrea gigas; Ecosystem; Energy Metabolism; Metabolomics; Nucleotides - metabolism; Plasma; Proteomics; Proteomics - methods; Transcriptomics; UVB exposure
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2022.160729

The increasing and intensifying ultraviolet B (UVB) radiation in sunlight is an environmental threat to aquatic ecosystems, potentially affecting the entire life cycle of wild or aquacultural Pacific oyster Crassostrea gigas with photoreception. Due to its complex composition, plasma is an important biological specimen for investigating the degree of disturbance from its steady state caused by the external environment in the open-pipe-type hemolymph of mollusks. We performed a multi-omic analysis of C. gigas plasma exposed to daylight UVB radiation. Hub differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) were identified using the functional classification of Clusters of Orthologous Groups of proteins (COGs) through the protein-protein interaction (PPI)-based maximal clique centrality (MCC) algorithm. Our results summarize three types of UVB influences (disruption of the cell membrane, promotion of nucleotide metabolism, and inhibition of energy metabolism) on C. gigas based on transcriptomic, proteomic, and metabolomic analyses. The associated hub DEGs, DEPs (e.g., nucleoside diphosphate kinase, malate dehydrogenase, and hydroxyacyl-coenzyme A dehydrogenase), and metabolites (e.g., uridine, adenine, deoxyguanosine, guanosine, and xylitol) in the plasma were identified as biomarkers of mollusk response to UVB radiation, and could be used to evaluate the influence of environmental UVB on mollusks in future studies. [Display omitted] •Using the plasma for evaluating the influence of UVB.•UVB ruptures cell membranes.•UVB promotes nucleotide metabolism.•UVB inhibits energy metabolism.