Identification of genomic regions governing moisture and heat stress tolerance employing association mapping in rice (Oryza sativa L.)

• Published in: Molecular biology reports 2023-02, Vol.50 (2), p.1499-1515
• Main Authors: Kommana, Madhavilatha, Reddy, D. Mohan, Amarnath, K., Naik, M. Vinod Kumar, Withanawasam, D. M., Bommisetty, Reddyyamini, Maneesha, K., Bhargavi, M., Eragam, Aparna, Reddy, B. V. Bhaskara, Sudhakar, P., Krishna, Lalam, Lekkala, Sivarama P., Chakravartty, Navajeet, Lachagari, V. B. Reddy, Vemireddy, Lakshminarayana R.
• Format: Article
• Language: English
• Subjects: Agricultural production; Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Chromosome 6; Chromosome Mapping - methods; Data analysis; Drought resistance; Environmental stress; Gene mapping; Genomics; Genotypes; Germplasm; Heat stress; Heat-Shock Response - genetics; Histology; Life Sciences; Linkage disequilibrium; Morphology; Original Article; Oryza - genetics; Oryza sativa; Phenotype; Plant Breeding; Quantitative trait loci; Recombination; Rice; Single-nucleotide polymorphism; Stress analysis
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-022-08153-y

Background Rice crop is damaged extremely by abiotic stress world-wide. The best approach to enhance drought tolerance in rice varieties is to identify and introgress yield QTLs with major effects. The Association mapping approach helps in the identification of genomic regions governing physiological, yield and yield attributes under moisture and heat stress conditions in diverse collections of crop germplasm, based on historic recombination events and linkage disequilibrium across the genome. Methods and Results The association mapping panel of 110 rice germplasm lines exhibited significant variation for all the traits in both irrigated and moisture stress conditions. The extent of yield reduction ranged to 83% during rabi , 2018–19, 53% in rabi , 2019–20 and 68% in pooled analysis. The genotypes Badami, Badshabhog, Pankaj, Varalu, Vasundhara, Vivekdhan, Krishna and Minghui63 exhibited drought tolerance with least yield penalty under moisture stress conditions. The genotypes Konark, MTU3626, NLR33671, PR118 and Triguna exhibited minimal reduction in heat stress tolerance traits. Association mapping of germplasm using 37808 SNP markers detected a total of 10 major MTA (Marker-trait association) clusters distributed on chromosomes 1, 3, 4 and 11 through mixed linear model (MLM) governing multiple traits from individual data analysis which are consistent across the years and situations. The pooled data generated a total of five MTA clusters located on chromosome 6. In addition, several novel unique MTAs were also identified. Heat stress analysis generated a total of 23 MTAs distributed on chromosomes 1, 5, 6 and 11. Candidate gene analysis detected a total of 53 and 38 genes under individual and pooled data analysis for various yield and yield attributes under control and moisture stress conditions, respectively and a total of 11 candidate genes in heat stress Conditions. Conclusion The major and novel MTAs identified in the present investigation for various drought and heat tolerant traits can be utilized for breeding climate-resilient rice varieties. The candidate genes predicted for key MTAs are of great value to deploy into the rice breeding after functional characterization.