Molting-related proteases in the brown planthopper, Nilaparvata lugens

• Published in: Insect biochemistry and molecular biology 2023-01, Vol.152, p.103893-103893, Article 103893
• Main Authors: Xie, Yu-Cheng, Zhang, Hou-Hong, Li, Han-Jing, Zhang, Xiao-Ya, Luo, Xu-Mei, Jiang, Ming-Xing, Zhang, Chuan-Xi
• Format: Article
• Language: English
• Subjects: Aminopeptidases - genetics; Aminopeptidases - metabolism; Animals; Ecdysis; Hemiptera - metabolism; Insect Proteins - metabolism; Metallocarboxypeptidase; Molting; Molting - genetics; Nilaparvata lugens; Procuticle; RNA Interference; Serine Proteases - metabolism; Trypsin; Trypsin - metabolism
• ISSN: 0965-1748; 1879-0240
• DOI: 10.1016/j.ibmb.2022.103893

Digestion and absorption of old cuticles during insect molting are necessary for new cuticle formation, during which complicated enzyme catalysis is essential. To date, a few carboxypeptidases, aminopeptidases and serine proteases (mostly trypsins) connected with cuticle digestion, zymogen activation and histological differentiation during the ecdysis of lepidopteran, dipteran and hymenopteran insects have been identified. However, little is known about these proteins in hemimetabolous insects. In this study, we identified 33 candidate trypsin and trypsin-like homologs, 14 metallocarboxypeptidase and 32 aminopeptidase genes in the brown planthopper Nilaparvata lugens, a hemipteran rice pest. Among the proteins encoded by these genes, 9 trypsin-like proteases, 3 metallocarboxypeptidases and 1 aminopeptidase were selected as potential procuticle hydrolases by bioinformatics analysis and in vivo validation. RNA interference targeting these genes demonstrated that 3 trypsin-like proteases (NlTrypsin-8, NlTrypsin-29 and NlTrypsin-32) genes and 1 metallocarboxypeptidase (NlCpB) gene were found to be essential for ecdysis in N. lugens; specifically, gene silencing led to incomplete cuticle degradation and arrested ecdysis, causing lethal morphological phenotype acquisition. Spatiotemporal expression profiling by quantitative PCR and western blotting revealed their specific expression in the integument and their periodic expression during each stadium, with a peak before ecdysis and eclosion. Transmission electron microscopy demonstrated corresponding ultrastructural defects after RNAi targeting, with NlCpB-silenced specimens having the most undigested old procuticles. Immunohistochemical staining revealed that NlTrypsin-8, NlTrypsin-29 and NlCpB were predominantly located in the exuvial space. This research further adds to our understanding of proteases and its potential role in insect ecdysis. [Display omitted] •33 trypsin, 14 metallocarboxypeptidase and 32 aminopeptidase genes were identified in the planthopper.•NlTrypsin-8, NlTrypsin-29, NlTrypsin-32 and NlCpB are integument-specific and periodic expression during each stadium.•Knockdown of NlTrypsin-8, NlTrypsin-29, NlTrypsin-32 and NlCpB caused lethal phenotype during ecdysis and eclosion.•NlTrypsin-8, NlTrypsin-29 and NlCpB located in between the new emerging cuticle and the old procuticle.