Confining the Sol‐Gel Reaction at the Water/Oil Interface: Creating Compartmentalized Enzymatic Nano‐Organelles for Artificial Cells

Living organisms compartmentalize their catalytic reactions in membranes for increased efficiency and selectivity. To mimic the organelles of eukaryotic cells, we develop a mild approach for in situ encapsulating enzymes in aqueous‐core silica nanocapsules. In order to confine the sol‐gel reaction a...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2023-03, Vol.62 (11), p.e202216966-n/a
Main Authors: Gonçalves, Jenifer Pendiuk, Promlok, Duangkamol, Ivanov, Tsvetomir, Tao, Shijia, Rheinberger, Timo, Jo, Seong‐Min, Yu, Yingjie, Graf, Robert, Wagner, Manfred, Crespy, Daniel, Wurm, Frederik R., Caire da Silva, Lucas, Jiang, Shuai, Landfester, Katharina
Format: Article
Language:English
Subjects:
Alkoxysilanes
Artificial Cell
Artificial Cells
Cascade chemical reactions
Catalysis
Catalysts
Encapsulation
Enzyme Encapsulation
Enzymes
Hollow Silica Nanoparticle
Nanocapsules
Nanoreactor
Organelles
Polymer Vesicle
Polymers
Proteolysis
Selectivity
Silica
Silicon Dioxide
Sol-gel processes
Water
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Summary:Living organisms compartmentalize their catalytic reactions in membranes for increased efficiency and selectivity. To mimic the organelles of eukaryotic cells, we develop a mild approach for in situ encapsulating enzymes in aqueous‐core silica nanocapsules. In order to confine the sol‐gel reaction at the water/oil interface of miniemulsion, we introduce an aminosilane to the silica precursors, which serves as both catalyst and an amphiphilic anchor that electrostatically assembles with negatively charged hydrolyzed alkoxysilanes at the interface. The semi‐permeable shell protects enzymes from proteolytic attack, and allows the transport of reactants and products. The enzyme‐carrying nanocapsules, as synthetic nano‐organelles, are able to perform cascade reactions when enveloped in a polymer vesicle, mimicking the hierarchically compartmentalized reactions in eukaryotic cells. This in situ encapsulation approach provides a versatile platform for the delivery of biomacromolecules. To mimic the compartmentalization of enzyme reactions in living organisms, in this study we developed an efficient and safe method for in situ encapsulating enzymes in aqueous‐core mesoporous nanocapsules. The enzyme‐carrying nanocapsules, as synthetic nano‐organelles, were able to successfully perform cascade reactions when enveloped in a polymer vesicle, mimicking the hierarchically compartmentalized reactions in the eukaryotic cells.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.202216966