Melatonin treatment delays postharvest senescence of broccoli with regulation of carotenoid metabolism

[Display omitted] •Melatonin treatment maintained the sensory quality of postharvest broccoli florets.•Melatonin induced the enrichment of endogenous melatonin in broccoli florets.•Melatonin down-regulated expression of carotenoid metabolism-related genes.•Melatonin inhibited contents of β-carotene,...

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Bibliographic Details
Published in:Food chemistry 2023-05, Vol.408, p.135185-135185, Article 135185
Main Authors: Lou, Jiajun, Wu, Chenghao, Wang, Hongfei, Cao, Shifeng, Wei, Yingying, Chen, Yi, Jiang, Shu, Shao, Xingfeng, Xu, Feng
Format: Article
Language:English
Subjects:
Abscisic Acid - metabolism
Brassica - metabolism
Broccoli
Carotenoid metabolism
Carotenoids - metabolism
Humans
Melatonin
Melatonin - metabolism
Time-to-Treatment
Yellowing
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Summary:[Display omitted] •Melatonin treatment maintained the sensory quality of postharvest broccoli florets.•Melatonin induced the enrichment of endogenous melatonin in broccoli florets.•Melatonin down-regulated expression of carotenoid metabolism-related genes.•Melatonin inhibited contents of β-carotene, β-cryptoxanthin, zeaxanthin and lutein. The effect of melatonin treatment on the carotenoid metabolism in broccoli florets during storage was explored. The results indicated that 100 µmol/L of melatonin maintained the sensory quality of broccoli florets, which retarded the increase of the L* value and the decrease of the H value. Melatonin treatment increased the activities of tryptophan decarboxylase (TDC), tryptamine 5-hydroxylase (T5H), serotonin N-acetyltransferase (SNAT) and N-acetylserotonin methyltransferase (ASMT), leading to the enrichment of endogenous melatonin content in broccoli florets. Meanwhile, the treatment inhibited the concentrations of β-carotene, β-cryptoxanthin, zeaxanthin and lutein, which was beneficial in delaying the yellowing of broccoli. In addition, a series of carotenoid biosynthetic genes such as BoPSY, BoPDS, BoZDS, BoLCYβ and BoZEP was also suppressed by melatonin. Further analysis revealed that the lower carotenoid content and the down-regulated BoNCED expression in treated broccoli resulted in less accumulation of abscisic acid precursors, inhibiting abscisic acid production during the yellowing process.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2022.135185