New experimental model of kidney injury: Photothrombosis-induced kidney ischemia

• Published in: Biochimica et biophysica acta. Molecular basis of disease 2023-03, Vol.1869 (3), p.166622-166622, Article 166622
• Main Authors: Brezgunova, Anna A., Andrianova, Nadezda V., Popkov, Vasily A., Tkachev, Sergey Y., Manskikh, Vasily N., Pevzner, Irina B., Zorova, Ljubava D., Timashev, Peter S., Silachev, Denis N., Zorov, Dmitry B., Plotnikov, Egor Y.
• Format: Article
• Language: English
• Subjects: Acute Kidney Injury - pathology; Bengal rose; Experimental model; Fibrosis; Humans; Ischemia - pathology; Kidney; Kidney - pathology; Photo-induced damage; Regeneration; Renal Insufficiency, Chronic - etiology; Renal Insufficiency, Chronic - pathology; Reperfusion Injury - pathology; X-Ray Microtomography - adverse effects
• ISSN: 0925-4439; 1879-260X
• DOI: 10.1016/j.bbadis.2022.166622

Acute kidney injury (AKI) is a frequent pathology with a high mortality rate after even a single AKI episode and a great risk of chronic kidney disease (CKD) development. To get insight into mechanisms of the AKI pathogenesis, there is a need to develop diverse experimental models of the disease. Photothrombosis is a widely used method for inducing ischemia in the brain. In this study, for the first time, we described photothrombosis-induced kidney ischemia as an appropriate model of AKI and obtained comprehensive characteristics of the photothrombotic lesion using micro-computed tomography (micro-CT) and histological techniques. In the ischemic area, we observed destruction of tubules, the loss of brush border and nuclei, connective tissue fibers disorganization, leukocyte infiltration, and hyaline casts formation. In kidney tissue and urine, we revealed increased levels in markers of proliferation and injury. The explicit long-term consequence of photothrombosis-induced kidney ischemia was renal fibrosis. Thus, we establish a new low invasive experimental model of AKI, which provides a reproducible local ischemic injury lesion. We propose our model of photothrombosis-induced kidney ischemia as a useful approach for investigating AKI pathogenesis, studying the mechanisms of kidney regeneration, and development of therapy against AKI and CKD. [Display omitted] •A model of focal ischemic kidney injury using photothrombosis was developed.•Kidney photothrombosis reproduces all conventional features of ischemic AKI.•The model provides clear ischemic locus separation.