Smartphone-based microplate reader for high-throughput quantitation of disease markers in serum

Herein, a smartphone-based portable reader with integrated optics for standard microtiter plates (96 wells) has been designed and demonstrated for high-throughput quantitation of validated biomarkers in serum. The customized optical attachment was simply constructed with a convex lens and a light so...

Full description

Saved in:
Bibliographic Details
Published in:Analyst (London) 2023-02, Vol.148 (4), p.735-741
Main Authors: Deng, Rong, Chao, Xiaoxin, Li, Haiqin, Li, Xiaochun, Yang, Zehua, Yu, Hua-Zhong
Format: Article
Language:English
Subjects:
Applications programs
Biomarkers
Colorimetry
Colorimetry - methods
Customization
Integrated optics
Laboratory equipment
Light sources
Mobile Applications
Mobile computing
Point-of-Care Systems
Reproducibility of Results
Smartphone
Smartphones
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Herein, a smartphone-based portable reader with integrated optics for standard microtiter plates (96 wells) has been designed and demonstrated for high-throughput quantitation of validated biomarkers in serum. The customized optical attachment was simply constructed with a convex lens and a light source, by which the transmitted light through a 96-well microtiter plate was converged for imaging with a smartphone, so that accurate and wide-range reading of the plate can be achieved. More importantly, relying on the digitized colorimetric analysis of the obtained images, concentrations of various biomarkers can be determined directly using the customized mobile app. A set of validated biomarkers for inflammation and infection, C-reactive protein (CRP), serum amyloid A (SAA), and procalcitonin (PCT) have been quantitated with this new system; both the response ranges and limits of detection meet the requirement of clinical tests. The consistency with the results obtained using a commercial microplate reader proves its reliability and precision, augments its potential as a point-of-care diagnostic device for on-site testing or resource-limited settings. A smartphone-based portable reader with integrated optics and a customized app for standard microtiter plates (96 wells) has been designed, developed, and demonstrated for high-throughput quantitation of validated biomarkers in serum.
ISSN:0003-2654
1364-5528
DOI:10.1039/d2an01571d