Diversity of Antimicrobial Peptide Genes in Bacillus from the Andaman and Nicobar Islands: Untapped Island Microbial Diversity for Disease Management in Crop Plants

Taxonomic and functional characterization of a total of 90 bacterial isolates representing bulk and rhizosphere soils of diverse niches of Andaman and Nicobar Islands, India were carried out. Twelve bacterial isolates were found promising for the biological suppression of agriculturally important fu...

Full description

Saved in:
Bibliographic Details
Published in:Current microbiology 2023-01, Vol.80 (1), p.45-45, Article 45
Main Authors: Sakthivel, Krishnan, Manigundan, Kaari, Sharma, Sushil Kumar, Singh, Reena, Das, Manisha Mohan, Devi, Varsha, Gautam, Raj Kumar, Nakkeeran, Sevugapperumal, Kumar, Aundy
Format: Article
Language:English
Subjects:
Antiinfectives and antibacterials
Antimicrobial Peptides
Bacillus
Bacillus - genetics
Bacillus subtilis - genetics
Bacteria
Biological control
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Disease Management
Genes
Islands
Leafspot
Life Sciences
Microbiology
Microorganisms
Peptides
Plant bacterial diseases
Plant growth
Plants
Rhizosphere
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sequence analysis
Solubilization
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Taxonomic and functional characterization of a total of 90 bacterial isolates representing bulk and rhizosphere soils of diverse niches of Andaman and Nicobar Islands, India were carried out. Twelve bacterial isolates were found promising for the biological suppression of agriculturally important fungal and bacterial plant pathogens such as Ralstonia solanacearum, Xanthomonas oryzae pv. oryzae, and Colletotrichum gloeosporioides. The 16S rRNA gene sequence analysis revealed their identity as belonging to Bacillus subtilis, Bacillus amyloliquefaciens, and Lysinibacillus sphaericus. The isolates were positive for plant growth promotion (PGP) traits including siderophore production, and nutrient solubilization especially phosphorous, zinc, and potassium. Interestingly, the PCR test confirmed the presence of 62 antimicrobial peptides (AMP) biosynthesis genes specific to the genus Bacillus. Whilst all tested species of Bacillus harboured the bacD biosynthesis gene, the B. subtilis (Ba_Abi), and B. amyloliquefaciens (Ba_Abi) harboured the maximum AMP biosynthesis genes analysed in the study. Upon in planta evaluation, the biocontrol potential of the bacterial isolates against leaf spot disease of chilli was observed. The study culminated in the isolation and identification of diverse Bacillus species for exploitation as bioinoculants for plant health management programmes.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-022-03086-y