Comparison of Gelatin/Polylysine‐ and Silk Fibroin/SDF‐1α‐Coated Mesenchymal Stem Cell‐Seeded Intracranial Stents

• Published in: Macromolecular bioscience 2023-04, Vol.23 (4), p.e2200402-n/a
• Main Authors: Peng, Qichen, Guo, Ruimin, Zhou, Yangyang, Teng, Ruidi, Cao, Yulin, Mu, Shiqing
• Format: Article
• Language: English
• Subjects: Adhesion; Aneurysm; Aneurysms; bioactive coatings; Biocompatibility; Biological effects; Cardiovascular system; Cell adhesion; Cell adhesion & migration; Cell proliferation; Chemokine CXCL12 - pharmacology; Chemotaxis; Coatings; Fibroins - pharmacology; Gelatin; Gelatin - pharmacology; Healing; Humans; Implants; Inflammation; Inflammatory response; Intracranial Aneurysm - therapy; intracranial stents; Macrophages; Mesenchymal Stem Cells; Nickel titanides; Polylysine; Polylysine - pharmacology; Secretion; Silk; Silk fibroin; Stem cells; Stents; Substrates; surface modifications
• ISSN: 1616-5187; 1616-5195
• DOI: 10.1002/mabi.202200402

Endothelialization of the aneurysmal neck is essential for aneurysm healing after endovascular treatment. Mesenchymal stem cell (MSC)‐seeded stents can promote aneurysm repair. The biological effects of coated and uncoated nitinol intracranial stents seeded with MSCs on vascular cells and macrophage proliferation and inflammation are investigated. Two stent coatings that exert pro‐aggregation effects on MSCs via different mechanisms are examined: gelatin/polylysine (G/PLL), which enhances cell adhesion, and silk fibroin/SDF‐1α (SF/SDF‐1α), which enhances chemotaxis. The aim is to explore the feasibility of MSC‐seeded coated stents in the treatment of intracranial aneurysms. The G/PLL coating provides the highest cytocompatibility and blood compatibility substrate for MSCs and vascular cells and promotes cell adhesion and proliferation. Moreover, it enhances MSC secretion and regulation of vascular cell and macrophage proliferation and chemotaxis. Although the SF/SDF‐1α coating promotes MSC secretion and vascular cell chemotaxis, it induces a greater degree of macrophage proliferation, chemotaxis, and secretion of pro‐inflammatory factors. MSC‐seeded stents coated with G/PLL may benefit stent surface endothelialization and reduce the inflammatory response after endovascular treatment of intracranial aneurysm. These effects may improve aneurysm healing and increase the cure rate. A stent that is capable of inducing rapid surface endothelialization may promote aneurysm healing. Two stent coatings that exert pro‐aggregation effects on MSCs via different mechanisms are examined in vitro: gelatin/polylysine (G/PLL), which enhances cell adhesion, and silk fibroin/SDF‐1α (SF/SDF‐1α), which enhances chemotaxis. MSC‐seeded stents coated with G/PLL may benefit stent surface endothelialization and reduce the inflammatory response after endovascular treatment of intracranial aneurysm.