The earth-star basidiomycetous mushroom Astraeus odoratus produces polyhydroxyalkanoates during cultivation on malt extract

• Published in: Archives of microbiology 2023-01, Vol.205 (1), p.34-34, Article 34
• Main Authors: Anh, Dau Hung, Dumri, Kanchana, Yen, Le Thi Hoang, Punyodom, Winita
• Format: Article
• Language: English
• Subjects: Absorptivity; Agar; Agaricales; Astraeus; Basidiocarps; Basidiomycota; Biochemistry; Biomedical and Life Sciences; Bioplastics; Biotechnology; Carbon sources; Cell Biology; Cell culture; Cellulose; Chemical compounds; Cultivation; Ecology; Environmental studies; Exudation; Fourier transforms; Fungiculture; Gas chromatography; Glycerol; Hydroxybutyrates; Infrared spectroscopy; Life Sciences; Malt; Mass spectrometry; Mass spectroscopy; Microbial Ecology; Microbiology; Mushrooms; Nitrogen sources; Original Paper; Peptones; Poly-3-hydroxybutyrate; Polyesters; Polyhydroxyalkanoates; Polyhydroxyalkanoates - chemistry; Polyhydroxybutyrate; Polyhydroxybutyric acid; Porous materials; Rice straw; Scanning electron microscopy; Soybeans
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-022-03297-8

Polyhydroxyalkanoates (PHAs) including poly-3-hydroxybutyrate (P3HB) as secondary metabolisms were in vitro produced by the edible basidiomycetous mushroom Astraeus odoratus during its growth on malt agar extract. Various carbon and nitrogen sources containing cellulose, glucose, glycerol, rice straw powder, soybean meal and peptone were investigated for the growth of basidiomycetous mushrooms. During cultivation, the A. odoratus culture exudated the considerably extracellular fluid up to approx. 2.3 ml on 2% malt extract agar plate within 7 days. The chemical compounds of the exudated fluid were further investigated by Fourier-transform infrared spectroscopy (FTIR) and gas chromatography/mass spectrometry (GC/MS); and its morphology of the lyophilized sample was observed by scanning electron microscope (SEM). FTIR results showed the characteristic bands of OH at 3445 cm −1 , CH/CH 2 /symmetric CH 3 (stretch) at 2923 and 2852 cm −1 , C=O at 1730 cm −1 , asymmetric CH 3 (bend) at 1454 and 1414 cm −1 , C−O of COO– at 1396 cm −1 and C–O–C at 1223, 1160, 1116, 1058 and 1019 cm −1 which were similar to the absorptive characteristics of P3HB. Methyl ester derivatives of GC/MS results identified 7 compounds including: 3-hydroxybutanoic (monomer of PHB), aminobenzoic, salicylic, hexadecenoic, octadecadienoic, octadecenoic and octadecanoic acids. SEM images revealed a fibriform and porous materials. Hence, the occurrence of PHAs was first described in a basidiomycetous mushroom A. odoratus . Thus, PHAs could be found not only in bacteria and but also in basidiomycetous mushroom, which can be promising target for bioplastics and green environmental studies.