eCry3.1Ab-resistant Western Corn Rootworm Larval Midgut Epithelia Respond Minimally to Bt Intoxication

Insect resistance to toxins derived from Bacillus thuringiensis (Bt) is a major issue in agriculture. Resistance to Bt has been linked to the loss of toxin binding sites within the insect, changes within the gut microbiota, and midgut tissue regeneration. Histopathological documentation of intoxicat...

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Bibliographic Details
Published in:Journal of economic entomology 2023-02, Vol.116 (1), p.263-267
Main Authors: Paddock, Kyle J., Dellamano, Kyle, Hibbard, Bruce E., Shelby, Kent S.
Format: Article
Language:English
Subjects:
Animals
Bacillus thuringiensis
Bacillus thuringiensis - genetics
Bacterial Proteins - genetics
Bacterial Proteins - pharmacology
Biotechnology
Cell proliferation
Coleoptera - physiology
Corn
Diabrotica virgifera virgifera
Endotoxins - pharmacology
Food
Genetically engineered foods
Genetically modified crops
Genetically modified organisms
Hemolysin Proteins - genetics
Hemolysin Proteins - pharmacology
histology
Ingestion
insect pest management
Insecticide Resistance
INSECTICIDE RESISTANCE AND RESISTANCE MANAGEMENT
Insects
Intestinal microflora
Intoxication
Larva - physiology
Larvae
Microbiota (Symbiotic organisms)
Midgut
Pest Control, Biological
Pest resistance
Pesticide resistance
Plants, Genetically Modified
resistance management
Stem cells
Tissue engineering
Toxins
Transgenic plants
Vegetables
Zea mays - genetics
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Summary:Insect resistance to toxins derived from Bacillus thuringiensis (Bt) is a major issue in agriculture. Resistance to Bt has been linked to the loss of toxin binding sites within the insect, changes within the gut microbiota, and midgut tissue regeneration. Histopathological documentation of intoxication and resistance to Bt is lacking for rootworms in the genus Diabrotica (Coleoptera: Chrysomelidae), a major target of Bt corn. Here, we document the morphological response of both Bt-resistant and Bt-susceptible larval western corn rootworm, Diabrotica virgifera virgifera LeConte, to intoxication with eCry3.1Ab. Gut lumen structural differences are subtle between the two colonies when feeding on non-Bt corn. However, upon ingestion of Bt-corn roots, susceptible larvae develop symptoms indicative of gut disruption by Bt, whereas resistant larvae incur milder effects. Mild disruption of the peritrophic matrix and gut lumen is accompanied by stem cell proliferation that may lead to midgut tissue regeneration. These results help contextualize the multifaceted nature of Bt-resistance in western corn rootworm for the first time from a histopathological perspective.
ISSN:0022-0493
1938-291X
DOI:10.1093/jee/toac191