The development of a dental light curable PRFe‐loaded hydrogel as a potential scaffold for pulp‐dentine complex regeneration: An in vitro study

Aim The study aimed to develop a bicomponent bioactive hydrogel formed in situ and enriched with an extract of platelet‐rich fibrin (PRFe) and to assess its potential for use in pulp‐dentine complex tissue engineering via cell homing. Methodology A bicomponent hydrogel based on photo‐activated natur...

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Bibliographic Details
Published in:International endodontic journal 2023-04, Vol.56 (4), p.447-464
Main Authors: Noohi, Parisa, Abdekhodaie, Mohammad J., Saadatmand, Maryam, Nekoofar, Mohammad H., Dummer, Paul M. H.
Format: Article
Language:English
Subjects:
Angiogenesis
Biological activity
Cell Differentiation
Cell proliferation
Cell viability
Chitosan
Chitosan - pharmacology
Collagen
Cytology
Cytoskeleton
Dental Pulp
Dentin
Fibrin
Gene expression
Homing behavior
Hydrogels
Hydrogels - chemistry
Hydrogels - pharmacology
injectable hydrogel
Mechanical properties
methacrylated chitosan
methacrylated collagen
Mineralization
Phalloidin
Platelet-Rich Fibrin
Regeneration
Tissue Engineering
Tissue Scaffolds - chemistry
Viscoelasticity
visible light crosslinking
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Summary:Aim The study aimed to develop a bicomponent bioactive hydrogel formed in situ and enriched with an extract of platelet‐rich fibrin (PRFe) and to assess its potential for use in pulp‐dentine complex tissue engineering via cell homing. Methodology A bicomponent hydrogel based on photo‐activated naturally derived polymers, methacrylated chitosan (ChitMA) and methacrylated collagen (ColMA), plus PRFe was fabricated. The optimized formulation of PRFe‐loaded bicomponent hydrogel was determined by analysing the mechanical strength, swelling ratio and cell viability simultaneously. The physical, mechanical, rheological and morphological properties of the optimal hydrogel with and without PRFe were determined. Additionally, MTT, phalloidin/DAPI and live/dead assays were carried out to compare the viability, cytoskeletal morphology and migration ability of stem cells from the apical papilla (SCAP) within the developed hydrogels with and without PRFe, respectively. To further investigate the effect of PRFe on the differentiation of encapsulated SCAP, alizarin red S staining, RT‐PCR analysis and immunohistochemical detection were performed. Statistical significance was established at p 
ISSN:0143-2885
1365-2591
DOI:10.1111/iej.13882