Virulence-associated Genome Sequences of Pasteurella canis and Unique Toxin Gene Prevalence of P. canis and Pasteurella multocida Isolated from Humans and Companion Animals

Comparative analysis of virulence factors (VFs) between and are lacking, although both cause zoonotic infections. We determined the virulence-associated genome sequence characteristics of and assessed the toxin gene prevalence unique to among clinical isolates of and . We selected 10 and 16 whole-ge...

Full description

Saved in:
Bibliographic Details
Published in:Annals of laboratory medicine 2023-05, Vol.43 (3), p.263-272
Main Authors: Yoshida, Haruno, Kim, Jung-Min, Maeda, Takahiro, Goto, Mieko, Tsuyuki, Yuzo, Shibata, Sachiko, Shizuno, Kenichi, Okuzumi, Katsuko, Kim, Jae-Seok, Takahashi, Takashi
Format: Article
Language:English
Subjects:
Animals
Humans
Pasteurella multocida - genetics
Pets
Prevalence
Virulence - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Comparative analysis of virulence factors (VFs) between and are lacking, although both cause zoonotic infections. We determined the virulence-associated genome sequence characteristics of and assessed the toxin gene prevalence unique to among clinical isolates of and . We selected 10 and 16 whole-genome sequences (WGSs) from the National Center for Biotechnology database. The VFanalyzer tool was used to estimate -characteristic VFs. Amino acid sequences of VFs were compared with multiple-aligned sequences. The genome structure containing -characteristic and adjacent loci was compared to the corresponding genome structure. After designing primer sequences and assessing their accuracy, we examined the gene prevalence of the -characteristic VFs using PCR among clinical isolates of and . Using VFanalyzer, we found virulence-associated cytolethal distending toxin ( ) loci common to all WGSs that were not found in WGSs. Similarities in the multiple alignments of CdtA-CdtB-CdtC amino acid sequences were found among the 10 WGSs. Shared or similar loci around were identified between the and genome structures. The PCR-based prevalence differed for and clinical isolates. -specific prevalence was identified among clinical isolates. These three loci may be unique toxin genes and promising targets for the rapid identification of in clinical settings.
ISSN:2234-3806
2234-3814
DOI:10.3343/alm.2023.43.3.263