Virulence-associated Genome Sequences of Pasteurella canis and Unique Toxin Gene Prevalence of P. canis and Pasteurella multocida Isolated from Humans and Companion Animals

Comparative analysis of virulence factors (VFs) between and are lacking, although both cause zoonotic infections. We determined the virulence-associated genome sequence characteristics of and assessed the toxin gene prevalence unique to among clinical isolates of and . We selected 10 and 16 whole-ge...

Full description

Saved in:
Bibliographic Details
Published in:Annals of laboratory medicine 2023-05, Vol.43 (3), p.263-272
Main Authors: Yoshida, Haruno, Kim, Jung-Min, Maeda, Takahiro, Goto, Mieko, Tsuyuki, Yuzo, Shibata, Sachiko, Shizuno, Kenichi, Okuzumi, Katsuko, Kim, Jae-Seok, Takahashi, Takashi
Format: Article
Language:English
Subjects:
Animals
Humans
Pasteurella multocida - genetics
Pets
Prevalence
Virulence - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c344t-34cdb8441b69827e3a88e9d802f1412b50bcf17b5a90533f8bb4bdebee8481413
cites cdi_FETCH-LOGICAL-c344t-34cdb8441b69827e3a88e9d802f1412b50bcf17b5a90533f8bb4bdebee8481413
container_end_page 272
container_issue 3
container_start_page 263
container_title Annals of laboratory medicine
container_volume 43
creator Yoshida, Haruno
Kim, Jung-Min
Maeda, Takahiro
Goto, Mieko
Tsuyuki, Yuzo
Shibata, Sachiko
Shizuno, Kenichi
Okuzumi, Katsuko
Kim, Jae-Seok
Takahashi, Takashi
description Comparative analysis of virulence factors (VFs) between and are lacking, although both cause zoonotic infections. We determined the virulence-associated genome sequence characteristics of and assessed the toxin gene prevalence unique to among clinical isolates of and . We selected 10 and 16 whole-genome sequences (WGSs) from the National Center for Biotechnology database. The VFanalyzer tool was used to estimate -characteristic VFs. Amino acid sequences of VFs were compared with multiple-aligned sequences. The genome structure containing -characteristic and adjacent loci was compared to the corresponding genome structure. After designing primer sequences and assessing their accuracy, we examined the gene prevalence of the -characteristic VFs using PCR among clinical isolates of and . Using VFanalyzer, we found virulence-associated cytolethal distending toxin ( ) loci common to all WGSs that were not found in WGSs. Similarities in the multiple alignments of CdtA-CdtB-CdtC amino acid sequences were found among the 10 WGSs. Shared or similar loci around were identified between the and genome structures. The PCR-based prevalence differed for and clinical isolates. -specific prevalence was identified among clinical isolates. These three loci may be unique toxin genes and promising targets for the rapid identification of in clinical settings.
doi_str_mv 10.3343/alm.2023.43.3.263
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2757160888</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2757160888</sourcerecordid><originalsourceid>FETCH-LOGICAL-c344t-34cdb8441b69827e3a88e9d802f1412b50bcf17b5a90533f8bb4bdebee8481413</originalsourceid><addsrcrecordid>eNpNkcFq3DAQhkVoyIYkD9BL0LEXu5JG9mqPYWmzC4EsZNOrkOwxOFjSVrJL-059yMq72xBdZkD__89IHyGfOSsBJHw1gysFE1BKKKEUNVyQayFAFqC4_PTes3pB7lJ6Y_nUjIsVuyILqCspAdQ1-fujj9OAvsHCpBSa3ozY0kf0wSF9wZ_TfJVo6OjOpBGniMNgaGN8n6jxLX31fdbQffjd-9mGdBfxlzkmHl3lB_HHCDcNYx7XGrpNYThO7WJwdDM540_ydXCH7A2ePvjemSHdkssuF7w71xvy-v3bfr0pnp4ft-uHp6IBKccCZNNaJSW39UqJJYJRCletYqLjkgtbMdt0fGkrs2IVQKeslbZFi6hk_jsON-TLKfcQQ35dGrXrUzOv7TFMSYtlteQ1U0plKT9JmxhSitjpQ8y7xj-aMz1z0pmTnjnp3IPOnLLn_hw_WYftu-M_FfgHSWiRQA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2757160888</pqid></control><display><type>article</type><title>Virulence-associated Genome Sequences of Pasteurella canis and Unique Toxin Gene Prevalence of P. canis and Pasteurella multocida Isolated from Humans and Companion Animals</title><source>PubMed Central</source><creator>Yoshida, Haruno ; Kim, Jung-Min ; Maeda, Takahiro ; Goto, Mieko ; Tsuyuki, Yuzo ; Shibata, Sachiko ; Shizuno, Kenichi ; Okuzumi, Katsuko ; Kim, Jae-Seok ; Takahashi, Takashi</creator><creatorcontrib>Yoshida, Haruno ; Kim, Jung-Min ; Maeda, Takahiro ; Goto, Mieko ; Tsuyuki, Yuzo ; Shibata, Sachiko ; Shizuno, Kenichi ; Okuzumi, Katsuko ; Kim, Jae-Seok ; Takahashi, Takashi</creatorcontrib><description>Comparative analysis of virulence factors (VFs) between and are lacking, although both cause zoonotic infections. We determined the virulence-associated genome sequence characteristics of and assessed the toxin gene prevalence unique to among clinical isolates of and . We selected 10 and 16 whole-genome sequences (WGSs) from the National Center for Biotechnology database. The VFanalyzer tool was used to estimate -characteristic VFs. Amino acid sequences of VFs were compared with multiple-aligned sequences. The genome structure containing -characteristic and adjacent loci was compared to the corresponding genome structure. After designing primer sequences and assessing their accuracy, we examined the gene prevalence of the -characteristic VFs using PCR among clinical isolates of and . Using VFanalyzer, we found virulence-associated cytolethal distending toxin ( ) loci common to all WGSs that were not found in WGSs. Similarities in the multiple alignments of CdtA-CdtB-CdtC amino acid sequences were found among the 10 WGSs. Shared or similar loci around were identified between the and genome structures. The PCR-based prevalence differed for and clinical isolates. -specific prevalence was identified among clinical isolates. These three loci may be unique toxin genes and promising targets for the rapid identification of in clinical settings.</description><identifier>ISSN: 2234-3806</identifier><identifier>EISSN: 2234-3814</identifier><identifier>DOI: 10.3343/alm.2023.43.3.263</identifier><identifier>PMID: 36544338</identifier><language>eng</language><publisher>Korea (South)</publisher><subject>Animals ; Humans ; Pasteurella multocida - genetics ; Pets ; Prevalence ; Virulence - genetics</subject><ispartof>Annals of laboratory medicine, 2023-05, Vol.43 (3), p.263-272</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-34cdb8441b69827e3a88e9d802f1412b50bcf17b5a90533f8bb4bdebee8481413</citedby><cites>FETCH-LOGICAL-c344t-34cdb8441b69827e3a88e9d802f1412b50bcf17b5a90533f8bb4bdebee8481413</cites><orcidid>0000-0003-0899-2860 ; 0000-0002-2100-4516 ; 0000-0003-4131-2062 ; 0000-0003-0809-6222 ; 0000-0001-6025-0341 ; 0000-0001-5346-5037 ; 0000-0001-6443-5853 ; 0000-0002-1918-2727 ; 0000-0001-7443-2090 ; 0000-0002-9399-909X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36544338$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yoshida, Haruno</creatorcontrib><creatorcontrib>Kim, Jung-Min</creatorcontrib><creatorcontrib>Maeda, Takahiro</creatorcontrib><creatorcontrib>Goto, Mieko</creatorcontrib><creatorcontrib>Tsuyuki, Yuzo</creatorcontrib><creatorcontrib>Shibata, Sachiko</creatorcontrib><creatorcontrib>Shizuno, Kenichi</creatorcontrib><creatorcontrib>Okuzumi, Katsuko</creatorcontrib><creatorcontrib>Kim, Jae-Seok</creatorcontrib><creatorcontrib>Takahashi, Takashi</creatorcontrib><title>Virulence-associated Genome Sequences of Pasteurella canis and Unique Toxin Gene Prevalence of P. canis and Pasteurella multocida Isolated from Humans and Companion Animals</title><title>Annals of laboratory medicine</title><addtitle>Ann Lab Med</addtitle><description>Comparative analysis of virulence factors (VFs) between and are lacking, although both cause zoonotic infections. We determined the virulence-associated genome sequence characteristics of and assessed the toxin gene prevalence unique to among clinical isolates of and . We selected 10 and 16 whole-genome sequences (WGSs) from the National Center for Biotechnology database. The VFanalyzer tool was used to estimate -characteristic VFs. Amino acid sequences of VFs were compared with multiple-aligned sequences. The genome structure containing -characteristic and adjacent loci was compared to the corresponding genome structure. After designing primer sequences and assessing their accuracy, we examined the gene prevalence of the -characteristic VFs using PCR among clinical isolates of and . Using VFanalyzer, we found virulence-associated cytolethal distending toxin ( ) loci common to all WGSs that were not found in WGSs. Similarities in the multiple alignments of CdtA-CdtB-CdtC amino acid sequences were found among the 10 WGSs. Shared or similar loci around were identified between the and genome structures. The PCR-based prevalence differed for and clinical isolates. -specific prevalence was identified among clinical isolates. These three loci may be unique toxin genes and promising targets for the rapid identification of in clinical settings.</description><subject>Animals</subject><subject>Humans</subject><subject>Pasteurella multocida - genetics</subject><subject>Pets</subject><subject>Prevalence</subject><subject>Virulence - genetics</subject><issn>2234-3806</issn><issn>2234-3814</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNpNkcFq3DAQhkVoyIYkD9BL0LEXu5JG9mqPYWmzC4EsZNOrkOwxOFjSVrJL-059yMq72xBdZkD__89IHyGfOSsBJHw1gysFE1BKKKEUNVyQayFAFqC4_PTes3pB7lJ6Y_nUjIsVuyILqCspAdQ1-fujj9OAvsHCpBSa3ozY0kf0wSF9wZ_TfJVo6OjOpBGniMNgaGN8n6jxLX31fdbQffjd-9mGdBfxlzkmHl3lB_HHCDcNYx7XGrpNYThO7WJwdDM540_ydXCH7A2ePvjemSHdkssuF7w71xvy-v3bfr0pnp4ft-uHp6IBKccCZNNaJSW39UqJJYJRCletYqLjkgtbMdt0fGkrs2IVQKeslbZFi6hk_jsON-TLKfcQQ35dGrXrUzOv7TFMSYtlteQ1U0plKT9JmxhSitjpQ8y7xj-aMz1z0pmTnjnp3IPOnLLn_hw_WYftu-M_FfgHSWiRQA</recordid><startdate>20230501</startdate><enddate>20230501</enddate><creator>Yoshida, Haruno</creator><creator>Kim, Jung-Min</creator><creator>Maeda, Takahiro</creator><creator>Goto, Mieko</creator><creator>Tsuyuki, Yuzo</creator><creator>Shibata, Sachiko</creator><creator>Shizuno, Kenichi</creator><creator>Okuzumi, Katsuko</creator><creator>Kim, Jae-Seok</creator><creator>Takahashi, Takashi</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0899-2860</orcidid><orcidid>https://orcid.org/0000-0002-2100-4516</orcidid><orcidid>https://orcid.org/0000-0003-4131-2062</orcidid><orcidid>https://orcid.org/0000-0003-0809-6222</orcidid><orcidid>https://orcid.org/0000-0001-6025-0341</orcidid><orcidid>https://orcid.org/0000-0001-5346-5037</orcidid><orcidid>https://orcid.org/0000-0001-6443-5853</orcidid><orcidid>https://orcid.org/0000-0002-1918-2727</orcidid><orcidid>https://orcid.org/0000-0001-7443-2090</orcidid><orcidid>https://orcid.org/0000-0002-9399-909X</orcidid></search><sort><creationdate>20230501</creationdate><title>Virulence-associated Genome Sequences of Pasteurella canis and Unique Toxin Gene Prevalence of P. canis and Pasteurella multocida Isolated from Humans and Companion Animals</title><author>Yoshida, Haruno ; Kim, Jung-Min ; Maeda, Takahiro ; Goto, Mieko ; Tsuyuki, Yuzo ; Shibata, Sachiko ; Shizuno, Kenichi ; Okuzumi, Katsuko ; Kim, Jae-Seok ; Takahashi, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-34cdb8441b69827e3a88e9d802f1412b50bcf17b5a90533f8bb4bdebee8481413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Humans</topic><topic>Pasteurella multocida - genetics</topic><topic>Pets</topic><topic>Prevalence</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoshida, Haruno</creatorcontrib><creatorcontrib>Kim, Jung-Min</creatorcontrib><creatorcontrib>Maeda, Takahiro</creatorcontrib><creatorcontrib>Goto, Mieko</creatorcontrib><creatorcontrib>Tsuyuki, Yuzo</creatorcontrib><creatorcontrib>Shibata, Sachiko</creatorcontrib><creatorcontrib>Shizuno, Kenichi</creatorcontrib><creatorcontrib>Okuzumi, Katsuko</creatorcontrib><creatorcontrib>Kim, Jae-Seok</creatorcontrib><creatorcontrib>Takahashi, Takashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of laboratory medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoshida, Haruno</au><au>Kim, Jung-Min</au><au>Maeda, Takahiro</au><au>Goto, Mieko</au><au>Tsuyuki, Yuzo</au><au>Shibata, Sachiko</au><au>Shizuno, Kenichi</au><au>Okuzumi, Katsuko</au><au>Kim, Jae-Seok</au><au>Takahashi, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Virulence-associated Genome Sequences of Pasteurella canis and Unique Toxin Gene Prevalence of P. canis and Pasteurella multocida Isolated from Humans and Companion Animals</atitle><jtitle>Annals of laboratory medicine</jtitle><addtitle>Ann Lab Med</addtitle><date>2023-05-01</date><risdate>2023</risdate><volume>43</volume><issue>3</issue><spage>263</spage><epage>272</epage><pages>263-272</pages><issn>2234-3806</issn><eissn>2234-3814</eissn><abstract>Comparative analysis of virulence factors (VFs) between and are lacking, although both cause zoonotic infections. We determined the virulence-associated genome sequence characteristics of and assessed the toxin gene prevalence unique to among clinical isolates of and . We selected 10 and 16 whole-genome sequences (WGSs) from the National Center for Biotechnology database. The VFanalyzer tool was used to estimate -characteristic VFs. Amino acid sequences of VFs were compared with multiple-aligned sequences. The genome structure containing -characteristic and adjacent loci was compared to the corresponding genome structure. After designing primer sequences and assessing their accuracy, we examined the gene prevalence of the -characteristic VFs using PCR among clinical isolates of and . Using VFanalyzer, we found virulence-associated cytolethal distending toxin ( ) loci common to all WGSs that were not found in WGSs. Similarities in the multiple alignments of CdtA-CdtB-CdtC amino acid sequences were found among the 10 WGSs. Shared or similar loci around were identified between the and genome structures. The PCR-based prevalence differed for and clinical isolates. -specific prevalence was identified among clinical isolates. These three loci may be unique toxin genes and promising targets for the rapid identification of in clinical settings.</abstract><cop>Korea (South)</cop><pmid>36544338</pmid><doi>10.3343/alm.2023.43.3.263</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-0899-2860</orcidid><orcidid>https://orcid.org/0000-0002-2100-4516</orcidid><orcidid>https://orcid.org/0000-0003-4131-2062</orcidid><orcidid>https://orcid.org/0000-0003-0809-6222</orcidid><orcidid>https://orcid.org/0000-0001-6025-0341</orcidid><orcidid>https://orcid.org/0000-0001-5346-5037</orcidid><orcidid>https://orcid.org/0000-0001-6443-5853</orcidid><orcidid>https://orcid.org/0000-0002-1918-2727</orcidid><orcidid>https://orcid.org/0000-0001-7443-2090</orcidid><orcidid>https://orcid.org/0000-0002-9399-909X</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2234-3806
ispartof Annals of laboratory medicine, 2023-05, Vol.43 (3), p.263-272
issn 2234-3806
2234-3814
language eng
recordid cdi_proquest_miscellaneous_2757160888
source PubMed Central
subjects Animals
Humans
Pasteurella multocida - genetics
Pets
Prevalence
Virulence - genetics
title Virulence-associated Genome Sequences of Pasteurella canis and Unique Toxin Gene Prevalence of P. canis and Pasteurella multocida Isolated from Humans and Companion Animals
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T20%3A00%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Virulence-associated%20Genome%20Sequences%20of%20Pasteurella%20canis%20and%20Unique%20Toxin%20Gene%20Prevalence%20of%20P.%20canis%20and%20Pasteurella%20multocida%20Isolated%20from%20Humans%20and%20Companion%20Animals&rft.jtitle=Annals%20of%20laboratory%20medicine&rft.au=Yoshida,%20Haruno&rft.date=2023-05-01&rft.volume=43&rft.issue=3&rft.spage=263&rft.epage=272&rft.pages=263-272&rft.issn=2234-3806&rft.eissn=2234-3814&rft_id=info:doi/10.3343/alm.2023.43.3.263&rft_dat=%3Cproquest_cross%3E2757160888%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c344t-34cdb8441b69827e3a88e9d802f1412b50bcf17b5a90533f8bb4bdebee8481413%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2757160888&rft_id=info:pmid/36544338&rfr_iscdi=true