The influence of simulated body fluid composition on carbonated hydroxyapatite formation

The materials for bone and dental implants are tested in vitro using simulated body fluid (SBF). The composition of most used SBF differs from that of blood plasma by high content of Cl super(-) ions and lower content of HCO super(-) sub(3) ions. Considering the composition of bone-like apatite, whi...

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Bibliographic Details
Published in:Ceramics (Praha) 2002-01, Vol.46 (1), p.9-14
Main Authors: HELEBRANT, Ales, JONASOVA, Lenka, SANDA, Ludvik
Format: Article
Language:English
Subjects:
Applied sciences
Biological and medical sciences
Body fluids
Bone
Building materials. Ceramics. Glasses
Chemical industry and chemicals
Composition
Dental prostheses
Exact sciences and technology
Glasses
Ions
Medical sciences
Nucleation
Precipitation (chemical)
Solutions
Structure, analysis, properties
Supersaturation
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology. Biomaterials. Equipments
X ray diffraction analysis
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Summary:The materials for bone and dental implants are tested in vitro using simulated body fluid (SBF). The composition of most used SBF differs from that of blood plasma by high content of Cl super(-) ions and lower content of HCO super(-) sub(3) ions. Considering the composition of bone-like apatite, which contains carbonate ions, the test results could be influenced by this difference. The glass of system Na sub(2)O-CaO-SiO sub(2)-P sub(2)O sub(5) was leached in fluids of composition (in mmol/l): 142 Na super(+), 5 K super(+), 2.5 Ca super(2+), 1 Mg super(2+), 1 SO super(2) sub(4) super(-), 1 HPO super(2) sub(4) super(-), 136 (Cl super(-)+HCO sub(3) super(-)). The content of HCO super(-) sub(3) ranged between 5 and 27 mmol/l. The decrease of phosphorus concentration was observed after 2 days in all solutions indicating the creation of phosphate layer on the glass surface. X-ray diffraction (XRD) confirmed the various extent of apatite layer creation in different solutions. The intensity of XRD peaks seems to be more influenced by the initial solution supersaturation than by the supersaturation values during the interaction. This fact indicates that the nucleation could be the control mechanism of apatite precipitation. Both experimental and supersaturation results indicate that the SBF with increased amount of HCO super(-) sub(3) ions is more appropriate and sensible to in vitro testing of bioactive materials.
ISSN:0862-5468