Microbial community dynamics responding to nutrient allocation associated with soybean cultivar ‘Jake’ ozone adaptation

• Published in: The Science of the total environment 2023-03, Vol.864, p.161008-161008, Article 161008
• Main Authors: Zhang, Kaile, Zentella, Rodolfo, Burkey, Kent O., Liao, Hui-Ling, Tisdale, Ripley H.
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial-fungal networks; Chloroflexi; Climate-resilient soybean; Elevated ozone; Glycine max; Microbiota; Nutrient source-sink balance; Ozone - analysis; Soil; Soil Microbiology; Soil microbiomes
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2022.161008

Tropospheric ozone (O3), a major air pollutant, leads to significant global yield loss in soybean [Glycine max (L.) Merr.]. Soybean cultivar ‘Jake’ shows O3 resilient traits in above-ground organs, but the root system remains sensitive to elevated O3 (eO3). Changing carbon (C) and nitrogen (N) resource composition during eO3 stress suggests that eO3 presumably alters belowground soil microbial communities and their driven nutrient transformation. Yet, the responses of belowground microbes to eO3 and their feedback on nutrient cycling in ‘Jake’ are unknown. In this study, we holistically investigated soil microbial communities associated with C and N dynamics and bacterial-fungal inter-kingdom networks in the rhizosphere and bulk soil at different developmental stages of ‘Jake’ grown under sub-ambient O3 [charcoal-filtered (CF) air, 12 h mean: 20 ppb] or eO3 (12 h mean: 87 ppb). The results demonstrated eO3 significantly decreased fungal diversity and complexity of microbial networks at different ‘Jake’ developmental stages, whereas bacterial diversity was more tolerant to eO3 in both bulk soil and rhizosphere. In the bulk soil, no O3-responsive microbial biomarkers were found to be associated with C and N content, implying eO3 may stimulate niche-based processes during ‘Jake’ growth. In contrast, this study identified O3-responsive microbial biomarkers that may contribute to the N acquisition (Chloroflexales) and C dynamics (Caldilineales, Thermomicrobiales, and Hypocreales) in the rhizosphere, which may support the O3 resilience of the ‘Jake’ cultivar. However, further investigation is required to confirm their specific contributions by determining changes in microbial gene expression. Overall, these findings conduce to an expanding knowledge base that O3 induces temporal and spatial changes in the effects of microbial and nutrient networks in the O3-tolerant agriculture ecosystems. [Display omitted] •eO3 decreased fungal diversity, whereas bacterial diversity was more tolerant to eO3.•eO3 decreased complexity of microbial networks throughout ‘Jake’ development.•eO3 induces nutrient-based processes by impacting microbial networks during ‘Jake’ growth.